Skip to main content
NCBI home page
The EMBO Journal logoLink to The EMBO Journal
. 1983;2(12):2323–2326. doi: 10.1002/j.1460-2075.1983.tb01741.x

Cell surface glycolipids of transformed NIH 3T3 cells transfected with DNAs of human bladder and lung carcinomas.

S Tsuchiya, S Hakomori
PMCID: PMC555452  PMID: 6667678

Abstract

Neutral glycolipids and gangliosides of NIH 3T3 cells oncogenically transformed by transfection of DNAs from human lung carcinoma (Lx-1) and human bladder carcinoma (Ej) have been investigated. The chemical quantity and the degree of cell surface exposure of gangliotriaosylceramide (Gg3) were greatly enhanced in NIH 3T3 cells transformed by transfection of DNAs of either Lx-1 or Ej carcinoma cells. An identical but more conspicuous change in cell surface exposure of Gg3 was observed in BALB/c 3T3 cells transformed by murine sarcoma virus Kirsten strain, but the same glycolipid was absent in the original Lx-1 or Ej human carcinomas. The mechanism that defines the chemical quantity and the organization of glycolipids is controlled by multiple factors. These include not only the quantity but also the organization of glycosyl transferases and hydrolases in membranes. This also involves membrane dynamics regulated through a cytoskeletal-membrane conjunction which may determine the degree of glycolipid exposure at the cell surface. The similarity of the chemical and organizational change of a single glycolipid, Gg3, between 3T3 transformants by Kirsten murine sarcoma virus and those by transfection of human cancer DNAs may indicate a common biochemical basis triggered by activation of the oncogene.

Full text

PDF
2323

Images in this article

2324Fig. 1.
on p.2324
2325Fig. 2.
on p.2325
2325Fig. 3.
on p.2325

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop J. M. Cellular oncogenes and retroviruses. Annu Rev Biochem. 1983;52:301–354. doi: 10.1146/annurev.bi.52.070183.001505. [DOI] [PubMed] [Google Scholar]
  2. Brady R. O., Fishman P. H. Biosynthesis of glycolipids in virus-transformed cells. Biochim Biophys Acta. 1974 Sep 9;355(2):121–148. doi: 10.1016/0304-419x(74)90001-8. [DOI] [PubMed] [Google Scholar]
  3. Der C. J., Cooper G. M. Altered gene products are associated with activation of cellular rasK genes in human lung and colon carcinomas. Cell. 1983 Jan;32(1):201–208. doi: 10.1016/0092-8674(83)90510-x. [DOI] [PubMed] [Google Scholar]
  4. Der C. J., Krontiris T. G., Cooper G. M. Transforming genes of human bladder and lung carcinoma cell lines are homologous to the ras genes of Harvey and Kirsten sarcoma viruses. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3637–3640. doi: 10.1073/pnas.79.11.3637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ellis R. W., Defeo D., Shih T. Y., Gonda M. A., Young H. A., Tsuchida N., Lowy D. R., Scolnick E. M. The p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes. Nature. 1981 Aug 6;292(5823):506–511. doi: 10.1038/292506a0. [DOI] [PubMed] [Google Scholar]
  6. FOLCH J., ARSOVE S., MEATH J. A. Isolation of brain strandin, a new type of large molecule tissue component. J Biol Chem. 1951 Aug;191(2):819–831. [PubMed] [Google Scholar]
  7. Fishman P. H., Brady R. O., Bradley R. M., Aaronson S. A., Todaro G. J. Absence of a specific ganglioside galactosyltransferase in mouse cells transformed by murine sarcoma virus. Proc Natl Acad Sci U S A. 1974 Feb;71(2):298–301. doi: 10.1073/pnas.71.2.298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gahmberg C. G., Hakomori S. I. External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes. J Biol Chem. 1973 Jun 25;248(12):4311–4317. [PubMed] [Google Scholar]
  9. Gahmberg C. G., Hakomori S. Surface carbohydrates of hamster fibroblasts. I. Chemical characterization of surface-labeled glycosphingolipids and aspecific ceramide tetrasaccharide for transformants. J Biol Chem. 1975 Apr 10;250(7):2438–2446. [PubMed] [Google Scholar]
  10. Gahmberg C. G., Kiehn D., Hakomori S. Changes in a surface-labelled galactoprotein and in glycolipid concentrations in cells transformed by a temperature-sensitive polyoma virus mutant. Nature. 1974 Mar 29;248(447):413–415. doi: 10.1038/248413a0. [DOI] [PubMed] [Google Scholar]
  11. Hakomori S. I., Teather C., Andrews H. Organizational difference of cell surface "hematoside" in normal and virally transformed cells. Biochem Biophys Res Commun. 1968 Nov 25;33(4):563–568. doi: 10.1016/0006-291x(68)90332-x. [DOI] [PubMed] [Google Scholar]
  12. Hakomori S. I., Wyke J. A., Vogt P. K. Glycolipids of chick embryo fibroblasts infected with temperature-sensitive mutants of avian sarcoma viruses. Virology. 1977 Feb;76(2):485–493. doi: 10.1016/0042-6822(77)90231-8. [DOI] [PubMed] [Google Scholar]
  13. Hakomori S. Glycosphingolipids in cellular interaction, differentiation, and oncogenesis. Annu Rev Biochem. 1981;50:733–764. doi: 10.1146/annurev.bi.50.070181.003505. [DOI] [PubMed] [Google Scholar]
  14. Kannagi R., Nudelman E., Levery S. B., Hakomori S. A series of human erythrocyte glycosphingolipids reacting to the monoclonal antibody directed to a developmentally regulated antigen SSEA-1. J Biol Chem. 1982 Dec 25;257(24):14865–14874. [PubMed] [Google Scholar]
  15. Krontiris T. G., Cooper G. M. Transforming activity of human tumor DNAs. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1181–1184. doi: 10.1073/pnas.78.2.1181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Magnani J. L., Smith D. F., Ginsburg V. Detection of gangliosides that bind cholera toxin: direct binding of 125I-labeled toxin to thin-layer chromatograms. Anal Biochem. 1980 Dec;109(2):399–402. doi: 10.1016/0003-2697(80)90667-3. [DOI] [PubMed] [Google Scholar]
  17. McCoy M. S., Toole J. J., Cunningham J. M., Chang E. H., Lowy D. R., Weinberg R. A. Characterization of a human colon/lung carcinoma oncogene. Nature. 1983 Mar 3;302(5903):79–81. doi: 10.1038/302079a0. [DOI] [PubMed] [Google Scholar]
  18. Murray M. J., Shilo B. Z., Shih C., Cowing D., Hsu H. W., Weinberg R. A. Three different human tumor cell lines contain different oncogenes. Cell. 1981 Aug;25(2):355–361. doi: 10.1016/0092-8674(81)90054-4. [DOI] [PubMed] [Google Scholar]
  19. Nudelman E., Kannagi R., Hakomori S., Parsons M., Lipinski M., Wiels J., Fellous M., Tursz T. A glycolipid antigen associated with Burkitt lymphoma defined by a monoclonal antibody. Science. 1983 Apr 29;220(4596):509–511. doi: 10.1126/science.6836295. [DOI] [PubMed] [Google Scholar]
  20. Rosenfelder G., Young W. W., Jr, Hakomori S. I. Association of the glycolipid pattern with antigenic alterations in mouse fibroblasts transformed by murine sarcoma virus. Cancer Res. 1977 May;37(5):1333–1339. [PubMed] [Google Scholar]
  21. SVENNERHOLM L. THE GANGLIOSIDES. J Lipid Res. 1964 Apr;5:145–155. [PubMed] [Google Scholar]
  22. Saito T., Hakomori S. I. Quantitative isolation of total glycosphingolipids from animal cells. J Lipid Res. 1971 Mar;12(2):257–259. [PubMed] [Google Scholar]
  23. Santos E., Tronick S. R., Aaronson S. A., Pulciani S., Barbacid M. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes. Nature. 1982 Jul 22;298(5872):343–347. doi: 10.1038/298343a0. [DOI] [PubMed] [Google Scholar]
  24. Shih C., Padhy L. C., Murray M., Weinberg R. A. Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts. Nature. 1981 Mar 19;290(5803):261–264. doi: 10.1038/290261a0. [DOI] [PubMed] [Google Scholar]
  25. Warren L., Buck C. A., Tuszynski G. P. Glycopeptide changes and malignant transformation. A possible role for carbohydrate in malignant behavior. Biochim Biophys Acta. 1978 Sep 18;516(1):97–127. doi: 10.1016/0304-419x(78)90005-7. [DOI] [PubMed] [Google Scholar]
  26. Yogeeswaran G., Sheinin R., Wherrett J. R., Murray R. K. Studies on the glycosphingolipids of normal and virally transformed 3T3 mouse fibroblasts. J Biol Chem. 1972 Aug 25;247(16):5146–5148. [PubMed] [Google Scholar]
  27. Young W. W., Jr, Hakomori S. I. Therapy of mouse lymphoma with monoclonal antibodies to glycolipid: selection of low antigenic variants in vivo. Science. 1981 Jan 30;211(4481):487–489. doi: 10.1126/science.7455688. [DOI] [PubMed] [Google Scholar]
  28. Young W. W., Jr, MacDonald E. M., Nowinski R. C., Hakomori S. I. Production of monoclonal antibodies specific for two distinct steric portions of the glycolipid ganglio-N-triosylceramide (asialo GM2). J Exp Med. 1979 Oct 1;150(4):1008–1019. doi: 10.1084/jem.150.4.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Young W. W., Jr, Portoukalian J., Hakomori S. Two monoclonal anticarbohydrate antibodies directed to glycosphingolipids with a lacto-N-glycosyl type II chain. J Biol Chem. 1981 Nov 10;256(21):10967–10972. [PubMed] [Google Scholar]
  30. Yu R. K., Ledeen R. W. Gangliosides of human, bovine, and rabbit plasma. J Lipid Res. 1972 Sep;13(5):680–686. [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES