Table 1. Characteristics of the sHLA peptidomes recovered from serum and plasma of healthy donors D1 to D3.
Sample | Total number of peptides | % 8-11mers | % predicted bindersa) | Post translational modifications |
||||
---|---|---|---|---|---|---|---|---|
Oxidation (M) | Cysteinylation (C) | Deamidation (N) | N-terminal cyclization (Q) | Phosphorylation (S, T, Y) | ||||
D1 plasma | 1841 | 95.4 | 76.1 | 101 | 38 | 15 | 4 | 2 |
D1 serum | 1816 | 90.7 | 74.2 | 59 | 43 | 26 | 2 | 2 |
D2 plasma | 2031 | 95.1 | 75.8 | 137 | 58 | 20 | 6 | 3 |
D2 serum | 2033 | 92.1 | 75.4 | 151 | 52 | 16 | 3 | 2 |
D3 plasma | 2741 | 97.2 | 89.1 | 285 | 106 | 16 | 2 | 6 |
D3 serum | 2761 | 94.6 | 89.2 | 184 | 81 | 21 | 5 | 7 |
NetMHCpan-2.8 predicted affinity below 500 nM [9]