Abstract
The presence of circulating tumor cells (CTC) or microemboli (CTM) in the peripheral blood can theoretically anticipate malignancy of solid lesions in a variety of organs. We aimed to preliminarily assess this capability in patients with pulmonary lesions of suspected malignant nature.
We used a cell-size filtration method (ScreenCell) and cytomorphometric criteria to detect CTC/CTM in a 3 mL sample of peripheral blood that was taken just before diagnostic percutaneous CT-guided fine needle aspiration (FNA) or core biopsy of the suspicious lung lesion.
At least one CTC/CTM was found in 47 of 67 (70%) patients with final diagnoses of lung malignancy and in none of 8 patients with benign pulmonary nodules. In particular they were detected in 38 (69%) of 55 primary lung cancers and in 9 (75%) of 12 lung metastases from extra-pulmonary cancers. Sensitivity of CTC/CTM presence for malignancy was 70.1% (95%CI: 56.9-83.1%), specificity 100%, positive predictive value 100% and negative predictive value 28.6% (95%CI: 11.9-45.3%). Remarkably, the presence of CTC/CTM anticipated the diagnosis of primary lung cancer in 3 of 5 patients with non-diagnostic or inconclusive results of FNA or core biopsy, whereas CTC/CTM were not observed in 1 patient with sarcoidosis and 1 with amarthocondroma.
These results suggest that presently, due to the low sensitivity, the search of CTC/CTM cannot replace CT guided percutaneous FNA or core biopsy in the diagnostic work-up of patients with suspicious malignant lung lesions. However, the high specificity may as yet indicate a role in cases with non-diagnostic or inconclusive FNA or core biopsy results that warrants to be further investigated.
Keywords: circulating tumor cells, lung nodule, lung cancer, lung metastases, CT-guided fine needle aspiration.
Introduction
The term “liquid biopsy” refers to the search and characterization in the peripheral blood of both circulating tumor cells (CTC) or microemboli (CTM) and biomolecules, such as free circulating DNA and micro-RNA 1. “Liquid biopsy” is receiving growing attention in lung oncology because it can non-invasively and repeatedly provide valuable information that is crucial for prognosis and treatment modulation according to a personalized medicine approach 2-4.
The presence of CTC or CTM can theoretically anticipate malignancy of solid lesions detected in a variety of organs and hence have a diagnostic value. So far, this possibility has been investigated in patients with breast, gastric, pancreatic and prostatic lesions 5-8. Lung is one special organ as it frequently harbors primary or secondary cancer lesions. The differential diagnosis with a variety of benign lung lesions is a very common clinical problem that is usually addressed by percutaneous or transbronchial fine needle aspiration (FNA) or core biopsy 9,10.
In this study, we aimed to preliminarily determine the predictive value for malignancy of detection of CTC/CTM in patients with single or multiple suspicious pulmonary lesions.
Patients and methods
Patients selection
This prospective study recruited 75 consecutive patients undergoing diagnostic percutaneous CT-guided FNA or core biopsy of pulmonary lesions of suspected malignant nature at the Diagnostic and Interventional Radiology Unit of the Careggi University Hospital of Florence, Italy. The study was approved by the Local Ethical Committee (decision May 18, 2015; protocol number BIO 15.009) and each patient provided a written consent to participate to the study.
Eligible patients were those with single or multiple peripheral lung nodules or masses amenable of percutaneous CT-guided FNA or core biopsy for diagnostic purposes. We excluded patients who refused consent to blood sampling, those with a history in the last 5 years of pulmonary and extra-pulmonary malignancy or who had received chemo or radiation therapy. Moreover, to avoid the possibility of CTC delivery in the bloodstream as a result of tumor manipulation 11,12 we also excluded patients who had undergone diagnostic transbronchial FNA or core biopsy within 7 days before the percutaneous procedure.
Blood sample collection
In each patient, 7 mL of blood were collected in one 10 mL EDTA tube just before CT-guided FNA or core biopsy through the antecubital vein access routinely prepared before the procedure.
Percutaneous CT-guided FNA and core biopsy
Percutaneous CT-guided FNA biopsy of the target lung lesion was performed with a 25-23 gauge needle by one of four interventional radiologists on a 40 rows of detectors Fluoro-CT scanner (Sensation Open, Siemens, Erlangen, Germany) dedicated to interventional procedures. FN aspirates were processed and analyzed by one of the two expert cytopathologists with Rapid On Site Examination (ROSE) 9,13 and cytological diagnosis was made according to the 2015 lung cancer classification 10. Immunocytochemistry on FNA material for primary lung cancer was performed in 34 patients in whom typical morphological patterns for adenocarcinoma, squamous cell carcinoma or neuroendocrine tumors were not present. In particular on the FNA material, but not on the isolated CTC/CTM (see below), a limited panel of TTF1, P63 and CD56 markers to enable diagnosis of lung adenocarcinoma, squamous cell carcinoma or small cell carcinoma, respectively, was used. Moreover, in 5 patients we performed immunocytochemistry on FNA material to differentiate metastasis of colorectal cancer or breast cancer from primary lung cancer. We used a panel with TTF1, cytokeratin 7 and cytokeratin 20, estrogen and progesterone receptor for such a purpose.
In 20 cases, FNA was supplemented by core biopsy performed with a 20-16 gauge needle and the latter was evaluated with routine histological procedures.
CTC analysis
Blood samples were processed within 3 hours after collection and CTC analysis was performed according to a standardized procedure reported in a previous study dealing with a different series of patients, selected because of advanced lung cancer 14. For each patient, we prepared 2 filters corresponding to a 6 mL volume of blood. The second filter served as backup in case of technical failures due to filter contamination or incomplete filtration and was stained on demand.
Isolation
Blood was filtered by the ScreenCell Cyto filtration devices according to the procedure previously described 14, 15 that allows isolation by cell size and does not involve any biological characterization of the putative CTC. Before filtration and in order to lyse red blood cells, 3 mL of the blood sample were diluted in 4 mL of the proprietary dilution buffer, according to the manufacturer's instructions (ScreenCell FC dilution buffer; ScreenCell). After blood filtration, an additional 1.6 mL of PBS was filtered to remove red blood cell debris. Filtration was usually completed within approximately 3 minutes. The filter was then disassembled from the filtration module and allowed to air-dry.
Identification
After an average of 10 days since FNA or core biopsy (range 7-21 days), the filters were evaluated in consensus by the same two expert cytopathologists (C.M. and M.Mat.) who had performed ROSE. They were blind to both the identity of the patient and the FNA or core biopsy results.
Cytological assessment was conducted directly on the filter. The track-etched filters were stained with hematoxylin solution S (Merck KGaA), applied to the membrane for 2 minutes, and with Shandon eosin Y aqueous (Thermo Electron Corporation, Thermo Fisher Scientific Inc) for 30 seconds. For microscopic observation, the ScreenCell Cyto filter was placed on a standard microscopy glass slide, and a 7-mm circular cover slip (Menzel-Glaser) was laid on the filter with the appropriate mounting medium.
CTC were identified among the cells retained on filter according to the presence of at least four of the following cytomorphological criteria: nucleus size ≥16 μm, anisonucleosis (ratio > 0.5), high (>2:1) nuclear/cytoplasmic ratio, irregular nuclei and presence of three-dimensional sheets 14-16. CTM were defined as clusters formed by ≥3 CTC 17.
Diagnostic work-up and staging
Final diagnoses were based on the results of FNA or core biopsy. In case of non-diagnostic (due to insufficient material) or inconclusive (doubtful features) results of CT-guided FNA or core-biopsy, diagnosis was based on clinical, laboratory, CT follow-up data or surgical pathology results.
The clinical or pathologic stage of ultimately diagnosed lung cancers was determined according to the 2009 Staging System 18.
Statistical analysis
A basic statistical evaluation was performed with computation of sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of CTC/CTM presence with regard to the final diagnosis of malignant or benign pulmonary lesion.
Results
The demographic data, the results of percutaneous CT-guided FNA or core biopsy, of the search of CTC/CTM, and the final diagnoses of the target lung lesions in the 75 patients are detailed in Table 1. Final diagnosis of primary lung cancer was made in 55 (23 stage I, 6 stage II, 9 stage III and 17 stage IV) and of benign lung nodule in 8 patients. In particular, FNA or core biopsy demonstrated primary lung cancer in 55 patients (38 adenocarcinomas, 12 squamous cell carcinomas, 1 not otherwise specified non small cell lung cancer, 1 small cell lung cancer, 2 carcinoid tumors, 1 neuroendocrine tumor with malignant features), secondary lesions (1 urothelial, 3 head and neck, 4 intestinal, 2 breast, 1 pancreas, 1 neuroendocrine) in 12 patients and benign lesions in 8 patients (3 amarthocondroma, 3 inflammation, 1 abscess, 1 sarcoidosis). In particular, the percutaneous CT guided procedure was non-diagnostic in 4 patients and inconclusive in 1 (overall 5/75=6.7%). Surgical pathology and follow-up data established diagnosis of primary lung cancer (adenocarcinoma) in 3 cases and of sarcoidosis and amarthocondroma in 1 case each.
Table 1.
CTC/CTM and diagnoses in patients undergoing percutaneous CT-guided FNA or core biopsy.
| N. | Patient Age, Sex | FNAB | Core Biopsy | CTC | CTM | Stage | Final diagnosis |
|---|---|---|---|---|---|---|---|
| 1 | 81, M | Squamous cell ca | - | 3 | 1 | IIa | Squamous cell ca |
| 2 | 69, F | Adenocarcinoma | - | 2 | 3 | pT1aN2IIIa | Adenocarcinoma |
| 3 | 64, M | Squamous cell ca | - | 12 | Ia | Squamous cell ca | |
| 4 | 79, M | Adenocarcinoma | - | 2 | 4 | IV | Adenocarcinoma |
| 5 | 79, F | Adenocarcinoma | - | 8 | 2 | IV | Adenocarcinoma |
| 6 | 65, F | Mtx (Squamous cell ca) | - | 1 | 5 | - | Mtx (Squamous cell ca Head & Neck) |
| 7 | 69, F | Inflammatory cells | Inflammation | - | - | - | Inflammation |
| 8 | 74, F | Adenocarcinoma | - | 2 | 1 | IV | Adenocarcinoma |
| 9 | 66, M | Adenocarcinoma | Adenocarcinoma | 8 | 2 | IV | Adenocarcinoma |
| 10 | 87, M | Mtx (Urothelial ca) | - | - | - | - | Mtx (Urothelial ca) |
| 11 | 74, M | Squamous cell ca | - | 6 | 11 | IIIa | Squamous cell ca |
| 12 | 75, M | Adenocarcinoma | - | 6 | 23 | IV | Adenocarcinoma |
| 13 | 67, M | Adenocarcinoma | - | 6 | 2 | pT1bN0Ia | Adenocarcinoma |
| 14 | 83, M | Mtx (Squamous cell ca) | - | 6 | 4 | - | Mtx (Squamous cell ca Head & Neck) |
| 15 | 74, M | Inflammatory cells | Inflammation | - | - | - | Inflammation |
| 16 | 79, F | Adenocarcinoma | - | 4 | 3 | IIa | Adenocarcinoma |
| 17 | 74, M | Amarthocondroma | - | - | - | - | Amarthocondroma |
| 18 | 79, F | Adenocarcinoma | - | 9 | 4 | IIIa | Adenocarcinoma |
| 19 | 82, F | Adenocarcinoma | Insufficient material | - | 1 | IIIb | Adenocarcinoma |
| 20 | 81, M | Adenocarcinoma | - | 8 | 18 | IV | Adenocarcinoma |
| 21 | 64, F | Mtx (Squamous cell ca) | - | - | - | - | Mtx (Squamous cell ca Larynx) |
| 22 | 73, M | Insufficient material | - | - | - | - | Sarcoidosis |
| 23 | 75, M | Squamous cell ca | - | 2 | 5 | pT1aN0Ia | Squamous cell ca |
| 24 | 71, M | Adenocarcinoma | - | 5 | 9 | IV | Adenocarcinoma |
| 25 | 72, M | Adenocarcinoma | - | 8 | 2 | pT1bN0Ia | Adenocarcinoma |
| 26 | 65, M | Adenocarcinoma | - | - | 2 | pT1aN0Ia | Adenocarcinoma |
| 27 | 75, M | Squamous cell ca | - | 2 | - | IIIa | Squamous cell ca |
| 28 | 67, M | NSCLC NOS | - | - | 3 | IV | NSCLC NOS |
| 29 | 57, F | Adenocarcinoma | - | 5 | 1 | IIIa | Adenocarcinoma |
| 30 | 68, F | Adenocarcinoma | - | 4 | 3 | pT1aN0Ia | Adenocarcinoma |
| 31 | 78, M | Mtx (Intestinal Adenocarcinoma) | - | - | - | - | Mtx (Colon Adenocarcinoma) |
| 32 | 70, F | Adenocarcinoma | - | 4 | - | IV | Adenocarcinoma |
| 33 | 80, M | Insufficient material | Insufficient material | - | 2 | Ia | Adenocarcinoma* |
| 34 | 83, M | Adenocarcinoma | - | - | 1 | IV | Adenocarcinoma |
| 35 | 72, M | Adenocarcinoma | - | 5 | 10 | pT1bN0Ia | Adenocarcinoma |
| 36 | 83, F | Adenocarcinoma | - | - | 8 | IIIa | Adenocarcinoma |
| 37 | 75, M | Mtx (Intestinal Adenocarcinoma) | - | 2 | 4 | - | Mtx (Colon Adenocarcinoma) |
| 38 | 74, F | Adenocarcinoma | - | 4 | 1 | IV | Adenocarcinoma |
| 39 | 63, F | Adenocarcinoma | - | - | 1 | Ia | Adenocarcinoma |
| 40 | 73, F | Adenocarcinoma | Adenocarcinoma | 10 | 3 | IV | Adenocarcinoma |
| 41 | 49, F | Mtx (Breast ca) | - | 5 | 3 | Mtx (Breast ca) | |
| 42 | 81, M | Adenocarcinoma | - | 3 | 1 | Ia | Adenocarcinoma |
| 43 | 75, M | Inflammatory cells | Abscess | - | - | - | Abscess |
| 44 | 69, M | Adenocarcinoma | Adenocarcinoma | - | 3 | Ia | Adenocarcinoma |
| 45 | 51, M | Adenocarcinoma | - | 2 | 1 | Ia | Adenocarcinoma |
| 46 | 70, M | Squamous cell ca | - | - | - | pT2bN0IIa | Squamous cell ca |
| 47 | 71, M | Mtx (neuroendocrine ca) | - | 5 | 3 | - | Mtx (Neuroendocrine ca) |
| 48 | 84, M | Adenocarcinoma | Adenocarcinoma | - | - | IV | Adenocarcinoma |
| 49 | 74, M | Squamous cell ca | - | - | - | IV | Squamous cell ca |
| 50 | 64, M | Insufficient material | - | - | - | - | Amarthocondroma |
| 51 | 74, F | Squamous cell ca | - | - | - | IIIa | Squamous cell ca |
| 52 | 76, F | Adenocarcinoma | - | - | - | pT2aN0IIa | Adenocarcinoma |
| 53 | 85, F | Neuroendocrine malignant | Neuroendocrine malignant | - | - | IV-diffuse | Neuroendocrine malignant |
| 54 | 65, M | Adenocarcinoma | - | - | - | Ia | Adenocarcinoma |
| 55 | 83, M | Squamous cell ca | - | - | - | Ia | Squamous cell ca |
| 56 | 62, F | Adenocarcinoma | - | - | - | Ia | Adenocarcinoma |
| 57 | 68, F | Mtx (Breast ca) | - | 2 | 1 | - | Mtx (Breast ca) |
| 58 | 82, F | Adenocarcinoma | - | - | - | Ib | Adenocarcinoma |
| 59 | 76, M | Adenocarcinoma | - | - | - | Ia | Adenocarcinoma |
| 60 | 64, F | Neuroendocrine tumor | - | - | - | pT1aN0Ia | Typical carcinoid |
| 61 | 66, M | Carcinoid | Carcinoid | - | - | pT1aN0Ia | Typical carcinoid |
| 62 | 79, F | Insufficient material | Insufficient material | 5 | 2 | IV | Adenocarcinoma* |
| 63 | 75, F | Inconclusive | Inconclusive | 3 | 10 | pT2bN1IIb | Adenocarcinoma |
| 64 | 52, F | Mtx (Intestinal adenocarcinoma) | - | - | 4 | - | Mtx (Colon Adenocarcinoma) |
| 65 | 75, M | SCLC | - | 6 | 5 | - | SCLC |
| 66 | 83, M | NSCLC | Squamous cell ca | - | - | IIIa | Squamous cell ca |
| 67 | 29, M | Amarthocondroma | Amarthocondroma | - | - | - | Amarthocondroma |
| 68 | 72, F | Adenocarcinoma | - | - | - | Ia | Adenocarcinoma |
| 69 | 67, M | Mtx (Pancreatic Adenocarcinoma) | - | 2 | 1 | IV | Mtx (Pancreas Adenocarcinoma) |
| 70 | 55, F | Adenocarcinoma | Adenocarcinoma | - | 5 | Ib | Adenocarcinoma |
| 71 | 85, F | Squamous cell ca | Squamous cell ca | - | - | Ib | Squamous cell ca |
| 72 | 68, F | Inflammatory cells | Inflammation | - | - | - | Inflammation |
| 73 | 79, M | Mtx (intestinal Adenocarcinoma) | Mtx (intestinal Adenocarcinoma) | - | 3 | - | Mtx (Colon adenocarcinoma) |
| 74 | 75, M | Adenocarcinoma | - | - | 10 | Ib | Adenocarcinoma |
| 75 | 51, M | Squamous cell ca | Squamous cell ca | - | - | IIa | Squamous cell ca |
Ca = carcinoma; FNAB = Fine Needle Aspiration Biopsy; Mtx = metastasis; NSCLC NOS = Non Small Cell Lung Cancer Not Otherwise Specified; SCLC = Small Cell Lung Carcinoma. * Diagnosis made with Trans-Bronchial Needle Aspiration biopsy.
Overall, in 18 patients it was necessary to stain and evaluate the second filter due to technical problems of incomplete filtration, presence of white blood elements and Candida albicans contamination that obscured possible CTC presence.
Figure 1 graphically shows the results of CTC/CTM search and of FNA or core biopsy in the 75 patients. At least one CTC/CTM in 3 mL of blood was found in 47 of 67 (70%) patients with final diagnoses of lung malignancy and in none of 8 patients with benign pulmonary nodules. In particular they were detected in 38 (69%) of 55 primary lung cancers and in 9 (75%) of 12 lung metastases from extra-pulmonary cancers.
Figure 1.
Results of CTC/CTM search and percutaneous FNA or core biopsy in 75 patients.
Overall sensitivity of CTC/CTM presence for malignancy was 70.1% (95%CI: 56.9-83.1%), specificity 100%, PPV 100% and NPV 28.6% (95%CI: 11.9-45.3%).
Remarkably, the presence of CTC/CTM anticipated the diagnosis of primary lung cancer in 3 of 5 patients with non-diagnostic or inconclusive results of FNA or core biopsy, whereas CTC/CTM were not observed in 1 patient with sarcoidosis and 1 with amarthocondroma.
CTC/CTM were present in 17 of 29 (58%) patients with stage I-II and in 21 of 26 (80%) patients with stage III-IV primary lung cancer and in 31 of 38 (81.5%) patients with adenocarcinoma and in 5 of 12 (42%) patients with squamous cell carcinoma.
Discussion
Percutaneous CT-guided FNA and core biopsy are established diagnostic procedures for patients with peripheral nodule or mass of suspicious malignant nature 19. The 93.3% diagnostic yield of FNA (with ROSE) and core biopsy in our series is in line with previous data 9, 19.
So far the diagnostic role of CTC/CTM search for lung cancer has been scarcely explored. One study evaluated CTC/CTM search with the ISET filtration method to anticipate lung cancer development in patients with chronic obstructive pulmonary disease who were followed up with CT until lung nodules were detected 20. This clinical context is different from ours in which we investigated the capability of CTC/CTM to anticipate malignancy in patients with evidence of suspicious lung nodules or masses on CT. In particular while sensitivity is a major concern for studies addressing screening of lung cancer, specificity is the crucial feature requested to a diagnostic tool in patients with high suspicion of lung cancer.
In the present study, we used the ScreenCell Cyto filtration method that allows isolation by cell size and does not involve any biological characterization of the putative CTC to preliminarily explore the predictive value for malignancy of detection of CTC/CTM in a prospective consecutive series of patients undergoing diagnostic FNA or core biopsy of pulmonary lesions of suspected malignant nature. Notably, in all our patients the blood sample for CTC search was taken before the diagnostic FNA or core biopsy by using the venous access that is routinely prepared in order to aid for therapeutic intervention in case of systemic adverse effects during or after the FNA or core biopsy procedure (hypotension, etc.). This time point for blood sampling enables us to get rid of the possibility that manipulation of lung cancer in diagnostic procedures including FNA or core biopsy determines delivery of CTC in the blood stream 11, 12.
There is growing evidence that CTM do not derive from CTC conglomeration in the blood stream and rather represent cell aggregates originating in the tumor and entering the vasculature, possibly as the result of the surface expression of specific molecules promoting cellular adhesion 21, 22. CTM might be more capable of metastasizing as compared with single CTC 22. Moreover, the presence and number of CTM is a poor prognostic factor, in particular for patients with small cell lung cancer 23, 24. However, since we were interested here in the potential diagnostic predictive value rather than prognostic role of CTC and CTM, the present study considered their presence both separately and altogether.
In prior studies evaluating possible contribute of presence of CTC or CTM in cases with putative malignant lesions of solid primary tumors of pancreas, stomach, breast and prostate, the overall sensitivity for malignant lesions ranged between 42 and 86% 5-8, whereas specificity was higher and in the 91-100% range 5-8. Using antibodies for detection of CTC (Veridex® LLC; NJ, USA), Maestro et al. 25 found 2 or more CTCs/7.5 mL in 31.5% of 438 patients with several types of primary cancer (breast, colorectal, prostate) but in none of 106 healthy control subjects. The 62.3% of patients with metastatic disease had 2 or more CTCs/7.5 mL as compared to only 14.0% of those with localized disease. The presence of CTCs was correlated to stage in the three studied tumor types and no differences in the number of cells were found between them.
When we considered the whole series of our patients we obtained a sensitivity of 70% and specificity of 100%. Sensitivity was 69% in primary lung cancers (58% in stage I-II lesions and 80% in stage III-IV lesions) and 75% in metastases.
The 69% positivity of CTC/CTM in our series of 55 primary lung cancers of different type and stage is substantially in line with the 46-80% CTC prevalence reported in previous series of early or advanced non small cell lung cancer 14, 16, 17, 26, 27.
Sensitivity, specificity and positive and negative predictive values in our series indicate that CTC/CTM presence in peripheral blood samples may be a highly specific but rather insensitive marker of the malignant nature of pulmonary lesions scheduled for percutaneous CT-guided biopsy. The reasons why CTC/CTM presence is detectable in most but not all cases of lung cancers and metastases, implying low sensitivity of the method 28, are not established. CTC/CTM detection may be influenced by several factors, including performance of the detection method, intermittent blood release of CTC from the primary tumor, and a lymphatic rather than blood tumor cell release.
Lack of CTC/CTM in all our 8 patients with FNA or core biopsy diagnosed benign pathology may be of interest. In fact, although CTC or CTM usually do not occur in healthy subjects 25, 29, our data indicate a potential role of CTC search in the clinical setting. Circulating non-hematological cells (CNHCs) which can be misdiagnosed as CTC have been reported in patients without cancers, namely with benign thyroid, parathyroid and pancreas pathologies 29, 30. However, application of cytomorphological criteria developed to characterize the malignant or non-malignant nature of CNHCs can solve the issue 4. Obviously, data in larger cohorts of patients with benign pulmonary lesions are required to further assess specificity of CTC/CTM presence.
In our opinion, also the data we obtained in the 5 patients who had non-diagnostic or inconclusive results of percutaneous FNA or core biopsy are of note. In fact, they suggest that CTC/CTM presence can anticipate malignancy and may theoretically represent a useful clue in cases with non-diagnostic or inconclusive FNA or core biopsy.
We recognize the following limitations of our study. First, CTC/CTM detection based on cytomorphometric criteria entails some operator dependence. However, the reported inter-operator variability for CTC identification using the cytomorphometric criteria we adopted is low 16 and filter visual assessment was performed in consensus by the cytopathologists. Second, CTC or CTM detection per se is certainly not sufficient for diagnostic purposes. In particular we did not address the capability of CTC/CTM characterization to differentiate between primary lung cancer and metastatic lung nodules from extra-pulmonary tissues, which is fundamental for diagnostic purposes. From this point of view, as immunocytochemistry in the FNA or core biopsy is capable to establish the primary or secondary nature of the lung malignant lesion, it can be anticipated that a similar phenotypic characterization of CTC/CTM with specific markers may overcome this limitation. Immunocytochemistry characterization of the CTC and CTM isolated with filtration is possible and represents a rapidly growing field 4, 7, 8, 31-34, but it was not performed here. Alternatively, genomic and expression analysis of CTC can be useful for such a purpose 2.
In conclusion, our preliminary data suggest that presently, due to the low sensitivity, the search of CTC/CTM cannot replace CT guided percutaneous FNA or core biopsy in the diagnostic work-up of patients with suspicious malignant lung lesions. However, the high specificity may as yet indicate a role in cases with non-diagnostic or inconclusive FNA or core biopsy results to be further investigated.
Figure 2.
(A-B): CTM in a case (number 63) of inconclusive FNA and core biopsy. (A) CT shows a consolidation in the left upper lobe with air bronchogram and small bronchiectases. (B) Filter (Hematoxylin and Eosin staining, original magnification x1,000) showing a CTM formed by more than 20 CTC with variably increased and irregularly shaped nuclei and multiple 8 µm pores of the filter appearing as black dots. Surgical pathology demonstrated a stage IIb adenocarcinoma.
Acknowledgments
This work was supported by Ente Cassa di Risparmio di Firenze and Regione Toscana (“CYTOPEM” POR CREO FSE 2007-2013), by Associazione Italiana per la Ricerca sul Cancro (IG-2015 17691), and by EC H2020 FRAMEWORK PROGRAMME (Project's title: SPIDIA4P: SPIDIA for Personalized Medicine - Standardisation of generic Pre-analytical procedures for In-vitro DIAgnostics for Personalized Medicine-GA n° 733112).
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