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. 2012 Jul 11;28(3):282–290. doi: 10.1007/s12264-012-1242-6

Possible novel roles of poly(rC)-binding protein 1 in SH-SY5Y neurocytes: an analysis using a dynamic Bayesian network

Li-Rong Huo 1,2, Jian-Tao Liang 3, Jun-Hua Zou 2, Lan-Ying Wang 1, Qi Li 4, Xiao-Min Wang 1,✉
PMCID: PMC5560330  PMID: 22622828

Abstract

Objective

Poly(rC)-binding protein 1 (PCBP1) belongs to the heterogeneous nuclear ribonucleoprotein family and participates in transcriptional and translational regulation. Previous work has identified transcripts targeted by both knockdown and overexpression of PCBP1 in SH-SY5Y neuroblastoma cells using a microarray or ProteomeLab™ protein fractionation 2-dimensions (PF-2D) and quadrupole time-of-flight mass spectrometer. The present study aimed to further determine whether these altered transcripts from major pathways (such as Wnt signaling, TGF-β signaling, cell cycling, and apoptosis) and two other genes, H2AFX and H2BFS (screened by PF-2D), have spatial relationships.

Methods

The genes were studied by qRT-PCR, and dynamic Bayesian network analysis was used to rebuild the coordination network of these transcripts.

Results

PCBP1 controlled the expression or activity of the seven transcripts. Moreover, PCBP1 indirectly regulated MAP2K2, FOS, FST, TP53 and WNT7B through H2AFX or regulated these genes through SAT. In contrast, TP53 and WNT7B are regulated by other genes.

Conclusion

The seven transcripts and PCBP1 are closely associated in a spatial interaction network.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s12264-012-1242-6 and is accessible for authorized users.

Keywords: PCBP1, RNA interference, overexpression, Bayesian network

Electronic supplementary material

12264_2012_1242_MOESM1_ESM.zip (214.5KB, zip)

Supplementary material, approximately 214 KB.

Footnotes

These authors contributed equally to this work.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s12264-012-1242-6 and is accessible for authorized users.

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Supplementary Materials

12264_2012_1242_MOESM1_ESM.zip (214.5KB, zip)

Supplementary material, approximately 214 KB.

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