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. 2017 Jun 1;206(4):2007–2039. doi: 10.1534/genetics.117.203174

Figure 9.

Figure 9

Oocyte M-phase entry is not required for expression of SPN-4::GFP::3xFLAG. The expression of SPN-4::GFP::3xFLAG was examined by fluorescence and DIC microscopy in (A–H) mutant backgrounds or (I–L) after RNAi treatments as indicated. Strong SPN-4::GFP::3xFLAG expression is observed in small abnormal oocytes in the lin-41(tn1487ts) mutant background (strain DG4192) at both (A and B) 25° and (C and D) 22°. A premature M-phase-entry phenotype characteristic of lin-41(n2914) null mutants is not observed in lin-41(tn1487ts) proximal oocytes at 22° (Spike et al. 2014a). SPN-4::GFP::3xFLAG expression is observed in proximal oocytes in (E and F) unmated fog-2(oz40) females (strain DG4185) and (G and H) acy-4(ok1806) hermaphrodites (from strain DG4212). M-phase entry occurs infrequently in these genetic backgrounds. (K and L) Strong SPN-4::GFP::3xFLAG expression is observed after cdk-1(RNAi). Note, different exposure times were used to image the effects of control(RNAi) [100 msec in (J)] and cdk-1(RNAi) [50 msec in (L)]. Expression levels are somewhat higher in the cdk-1(RNAi) one-cell arrested embryos, possibly because they continue to express SPN-4::GFP::3xFLAG. Bar, 50 μm.