Table 2. Mean dN/dS, dN, dS, and Fop for the small subset of the most extremely high expressed (≥ 1000 FPKM) testis-biased and ovary-biased CDS in Aedes.
| Parameter | Testis-biased short | Ovary-biased short | Testis-biased long | Ovary-biased long | Unbiased short | Unbiased long |
|---|---|---|---|---|---|---|
| dN/dS | 0.098 (0.023)a | 0.061 (0.007)b | 0.118 (0.014)a | 0.083 (0.016)b | 0.034 (0.016)a | 0.083 (0.030) |
| dN | 0.065 (0.017)a | 0.040 (0.008)b | 0.084 (0.013)a | 0.053 (0.017)b | 0.016 (0.009)a | 0.021 (0.006) |
| dS | 0.577 (0.116)a | 0.566 (0.036)a | 0.631 (0.049)a | 0.503 (0.078)b | 0.285 (0.034)a | 0.279 (0.040) |
| Fop | 0.499 (0.027)a | 0.507 (0.011)a | 0.448 (0.0169)a | 0.507 (0.020)b | 0.617 (0.014)a | 0.621 (0.011) |
| N | 21 | 107 | 49 | 17 | 23 | 3 |
Standard errors are in parenthesis. Different letters for each parameter within each length class (a versus b) indicate a statistically significant difference using randomization tests (P < 0.05). As unbiased long CDS had N = 3, the parameter values were included for completeness, but were not used in randomization tests. dN, nonsynonymous substitution rate; dS, synonymous substitution rate; Fop, frequency of optimal codons.
a
The unbiased short CDS exhibit a statistically significant difference from testis- and ovary-biased CDS (short and long), P < 0.05.