Figure 1. SitA1 is the swarm inhibition determinant.
(A) sitBAI1 operon found on one of the Mx-alpha elements that was lost from DK1622. SS, signal sequence. (B) Swarm inhibition assays with indicated motile and nonmotile strains. White arrow illustrates swarm inhibition with control strains. NA, not applicable. Bar, 1 mm. (C) Expression of sitBAI2 in a non-antagonistic nonmotile background results in modest swarm inhibition (indicated by *) compared to sitBAI1+ (shown in B). Expression of sitBAI3 in the non-antagonistic nonmotile background results in complete swarm inhibition of ∆sitBAI3. Here and elsewhere see Supplementary file 2A for strain details.