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. 2015 Aug 8;31(4):480–490. doi: 10.1007/s12264-015-1547-3

Identification of autophagy signaling network that contributes to stroke in the ischemic rodent brain via gene expression

Kun Liang 1, Lei Zhu 1, Jinyun Tan 1, Weihao Shi 1, Qing He 1, Bo Yu 2,✉
PMCID: PMC5563718  PMID: 26254060

Abstract

Autophagy plays a vital role in cerebral ischemia and may be a potential target for developing novel therapy for stroke. In this study, we constructed an autophagy-related pathway network by analyzing the genes related to autophagy and ischemic stroke, and the risk genes were screened. Two autophagy-related modules were significantly up-regulated and clustered to influence cerebral ischemia. Besides, three key modular genes (NFKB1, RELA, and STAT3) were revealed. With 5-fold cross validation, the ROC curves of NFKB1, RELA, and STAT3 were 0.8256, 0.8462, and 0.8923. They formed a complex module and competitively mediated the activation of autophagy in cerebral ischemia. In conclusion, a module containing NFKB1, RELA, and STAT3 mediates autophagy, serving as a potential biomarker for the diagnosis and therapy of ischemic stroke.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s12264-015-1547-3 and is accessible for authorized users.

Keywords: cerebral ischemia, autophagy, functional module, stroke, gene expression profile

Electronic supplementary material

12264_2015_1547_MOESM1_ESM.pdf (86.3KB, pdf)

Supplementary material, approximately 75 KB.

Footnotes

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s12264-015-1547-3 and is accessible for authorized users.

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