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. 2017 Apr 10;8(29):47121–47135. doi: 10.18632/oncotarget.17004

Figure 2. Functional analysis of HOXB7 by siRNA inhibition.

Figure 2

(A) CCK8 assay showed that cell proliferation was significantly suppressed after HOXB7 siRNA1083 transfection for 48 and 72 hours. (B) Cell cycle analysis showed that HOXB7 siRNA1083 induced S phase arrest and increased the proportion of cells in the G0/G1 phase. (C) Treatment with siRNA1083 had no significant effect on apoptosis in HCCLM3 cells. (D) Wound healing assays revealed a significant delay in the wound closure rate of HCCLM3 siRNA1083 cells compared with control cells at 48 and 72 hours after incubated with 25 μg/ml mitomycin C (Sigma, M4287-2MG) for 3h. (E) In vitro migration and invasion assays showed that the migration and invasion of the HOXB7 siRNA1083-treated group was significantly lower than that of the control group at 48 and 72 hours respectively. *, P<0.05; **, P<0.01; ***, P<0.001.