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. 2017 Apr 10;8(29):47121–47135. doi: 10.18632/oncotarget.17004

Figure 4. Validation gene changes after HOXB7 siRNA and its interaction with bFGF.

Figure 4

(A and B) Microarray experiments identified several genes that were differentially expressed between the siRNA group and the control group, and these findings were validated by qRT-PCR. (C) Expression of bFGF, beta-catenin, and E-cadherin in the siRNA and control group by western blot. (D) Expression of p-ERK was up-regulated in MHCC97L-HOXB7 pCDNA3 cells and down-regulated in HCCLM3-pGCSIL-GFP-HOXB7 shRNA cells, and SU5402 was down-regulated the expression of p-ERK in MHCC97L-HOXB7 pCDNA3 cells. (E) bFGF promoter-specific PCR primers could amplify this promoter region from DNA that was immunoprecipitated with the anti-HOXB7 antibody but not with the nonimmune IgG. (F) HOXB7 and bFGF significantly increased SRE luciferase reporter activity, while SU5402 could restrain it. (***, P<0.001).