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. 2017 May 18;8(29):47801–47815. doi: 10.18632/oncotarget.17999

Figure 5. The transcriptional factor of Twist1 binding to the promoter region of VE-cadherin.

Figure 5

(A) ChIP assay of Twist1 and Twist2 interactions with the VE-cadherin promoter. Bands are PCR products targeting P1-P4 of the VE-cadherin promoter. Specific anti-Twist1, anti-Twist2, or control normal mouse IgG was used for immunoprecipitations, whereas genomic DNA was used as the input control. (B) The effect of Twist1 on the transcriptional activity of the VE-cadherin promoter. Mutated promoters that specifically delete P1 or P4 of the Twist1 binding elements were introduced into AsPC-1 cells. Firefly luciferase expression levels were normalized to the luciferase activity of the internal Renilla control. The results indicate that both P1 and P4 elements are positive regulatory elements. Separate experiments performed in triplicate for each group. One-way analysis of variance (ANOVA) was used, * = P<0.05.