Figure 2. Changes in body weight and adipose tissue deposition in WT and Rcan2^−/− mice after sham-operation or bilateral ovariectomy fed on a NCD.

(A) Growth curves of sham-operated (sham) females and ovariectomized (OVX) females fed NCD. Arrows show the time of sham-operation or ovariectomy. (B) Increased body weight measured from postnatal week 6 to week 18. (C) Mean weights of gonadal (GWAT) and retroperitoneal white adipose tissue (RWAT) and (D) mean tibia lengths in females at postnatal week 18. The number of mice (n) used in the experiments was indicated directly in each panel of figures. Statistics were performed using one-way ANOVA, and individual group differences presented here were measured using Bonferroni correction. *: p <0.05, **: p <0.01, ***: p <0.001. All values are given as means ± SEM.