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. 2017 Aug 19;19(9):716–733. doi: 10.1016/j.neo.2017.07.001

Figure 8.

Figure 8

Internalization of H3L3 and H2aL2a antibodies in 4 T1 and MDA-MB-231 cells. A) Internalization by EIA detection of antibody surface binding following incubation of cells with antibody at 4°C (no internalization) and 37°C degrees (to induce internalization). All antibodies displayed internalization, where H2aL2a displayed significantly more internalization than chimeric (*, P < .05) and H3L3 showing significantly more internalization compared to all antibodies (**, P < .05). Bars represent mean ± S.E. (n = 3 experiments). B) Representative images showing immunofluorescent staining of Ab H2aL2a and the lysosomal protein marker, LAMP-1 (green). Top panels MDA-MB-231 cells were pre-incubated with antibody (1ug/mL) for 20 min at 4°C. Lower panels after at 37°C for 60 min. Merged images confirm internalization of H2aL2a and lysosomal co-localization with LAMP-1 in merged images (arrows). Total magnification was 40×.