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. 2017 Aug 19;19(9):716–733. doi: 10.1016/j.neo.2017.07.001

Figure 9.

Figure 9

Characterization of Maytansine (DM1) Conjugate of H2aL2a. A) H2aL2a-DM1 conjugate production. HIC-HPLC Chromatogram of H2aL2a Ab and H2aL2a-DM1 conjugate at A280. B) In vitro cell efficacy against human breast cancer (BC) cell lines using H2aL2a-DM1. C) In vitro efficacy against human lung cancer cell lines using H2aL2a-DM1. NSCLC is a non-small cell lung cancer and SCLC is a small cell lung cancer. B) & C) DM1 conjugated H2aL2a antibody showed enhanced anti proliferative activity on TF-Ag positive breast and lung cancer cell lines. All experiments were carried out to Day 5 of treatment and cell viability detected using a Cell-Glo reagent. (IC50 values, μg/mL H2aL2a-DM1).