Table 1.

Antibodies used in this study

Antibody name	Species/isotype/immunogen	Manufacturer information	Concentration used
AMIGO-1, anti-AMIGO-1 rabbit pAb	Raised against aa 394–492 of mouse AMIGO-1 (cytoplasmic C-terminus).	Trimmer Lab. Rabbit 28330 RRID:AB_2571515	1:400 dilution of affinity purified pAb, concentration unknown
L98/12, anti-AMIGO-1 mouse IgG1 mAb	Raised against aa 28–370 of mouse AMIGO-1 (extracellular N-terminus).	Trimmer lab. RRID:AB_2571516	1:3 dilution of tissue culture supernatant, concentration unknown
K89/34, anti-Kv2.1 mouse IgG1 mAb	Raised against aa 837–853 of rat Kv2.1.	Trimmer lab. NeuroMab catalog 73-014 RRID:AB_10672253	5 µg/ml purified mAb
L61C/30, anti-Kv2.1 mouse IgG1 mAb	Raised against aa 595–616 of rat Kv2.1.	Trimmer lab. RRID:AB_2532100	1:5 dilution of tissue culture supernatant, concentration unknown
N372B/1, anti-Kv2.2 mouse IgG1 mAb	Raised against aa 717–907 of rat Kv2.2. Binds within aa 764–907. Species reactivity with mouse, rat, ferret, macaque and human	NeuroMab catalog 73–369, RRID:AB_2315869	1:3 dilution of tissue culture supernatant, concentration unknown
N372B/60, anti-Kv2.2 mouse IgG2b mAb	Raised against aa 717–907 of rat Kv2.2. Binds within aa 764–907. Species reactivity with mouse and rat	NeuroMab catalog 73–360, RRID:AB_2315867	1:10 dilution of tissue culture supernatant, concentration unknown
N372C/51, anti-Kv2.2 mouse IgG1 mAb	Raised against aa 717–907 of rat Kv2.2. Binds within aa 717–763 Species reactivity with mouse and rat	NeuroMab catalog 73–358, RRID:AB_2315865	1:2 dilution of tissue culture supernatant, concentration unknown
N133/21, anti-RGS14 mouse IgG2a mAb	Raised against aa 1–544 of rat RGS14.	NeuroMab catalog 73–170, RRID:AB_10698026	1:10 dilution of tissue culture supernatant, concentration unknown

Details of the polyclonal (pAb) and monoclonal (mAb) Abs used in this study.