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. Author manuscript; available in PMC: 2018 Aug 21.
Published in final edited form as: Analyst. 2017 Aug 21;142(17):3183–3193. doi: 10.1039/c7an00787f

Figure 2. Characterization of cross-reactivity of poly(NIPAM-co-MAA) nanogels.

Figure 2

Adsorption capacity values (Q) of the eleven proteins to R1-R5 nanogels were determined from equilibrium protein binding experiments performed in (a) 0.1X PBS and (b) 0.1X HBS with initial protein concentrations of 0.5 mg/mL. (c) Difference between Q for protein binding in the two buffers (QHBS – QPBS) showing the impact of buffer on protein binding behavior. Statistical analysis was performed to determine significant differences in Q in the two buffers (n=3, Šidák’s test, $= p<0.05, # = p<0.01, * = p<0.001). Red shades = low pI proteins (pI < 6.0), gray shades = near-neutral pI proteins (6.0 ≤ pI ≤ 8.0), blue shades = high pI proteins (pI > 8.0).