Skip to main content
NCBI home page
The EMBO Journal logoLink to The EMBO Journal
. 1984 Mar;3(3):563–568. doi: 10.1002/j.1460-2075.1984.tb01847.x

Transcription of the Drosophila white locus and some of its mutants.

V Pirrotta, C Bröckl
PMCID: PMC557387  PMID: 6425054

Abstract

The white locus produces one major, though rare, RNA of 2.6 kb, found throughout development. Minor species of lower mol. wt. are also produced. One of these is male specific. Transcription was analysed by Northern blot hybridisation in the wild-type and several mutants: wa, w67c23, we, wsp and zeste. These and the results of S1 mapping reveal the presence of at least four introns. One of these, a micro-intron of 30-50 nucleotides contains the site of copia insertion in the wa mutant.

Full text

PDF
563

Images in this article

563Fig. 1.
on p.563
563Fig. 2.
on p.563
565Fig. 4.
on p.565
565Fig. 5.
on p.565
566Fig. 6.
on p.566
566Fig. 7.
on p.566
567Fig. 8.
on p.567

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bingham P. M., Judd B. H. A copy of the copia transposable element is very tightly linked to the Wa allele at the white locus of D. melanogaster. Cell. 1981 Sep;25(3):705–711. doi: 10.1016/0092-8674(81)90177-x. [DOI] [PubMed] [Google Scholar]
  2. Bingham P. M., Levis R., Rubin G. M. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981 Sep;25(3):693–704. doi: 10.1016/0092-8674(81)90176-8. [DOI] [PubMed] [Google Scholar]
  3. Collins M., Rubin G. M. Structure of the Drosophila mutable allele, white-crimson, and its white-ivory and wild-type derivatives. Cell. 1982 Aug;30(1):71–79. doi: 10.1016/0092-8674(82)90013-7. [DOI] [PubMed] [Google Scholar]
  4. Gehring W. J., Paro R. Isolation of a hybrid plasmid with homologous sequences to a transposing element of Drosophila melanogaster. Cell. 1980 Apr;19(4):897–904. doi: 10.1016/0092-8674(80)90081-1. [DOI] [PubMed] [Google Scholar]
  5. Gelbart W. M., Wu C. T. Interactions of zeste mutations with loci exhibiting transvection effects in Drosophila melanogaster. Genetics. 1982 Oct;102(2):179–189. doi: 10.1093/genetics/102.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldberg M. L., Paro R., Gehring W. J. Molecular cloning of the white locus region of Drosophila melanogaster using a large transposable element. EMBO J. 1982;1(1):93–98. doi: 10.1002/j.1460-2075.1982.tb01130.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Green M. M. Controlling element mediated transpositions of the white gene in Drosophila melanogaster. Genetics. 1969 Feb;61(2):429–441. doi: 10.1093/genetics/61.2.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. JUDD B. H. Formation of duplication-deficiency products by asymmetrical exchange within a complex locus of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1961 Apr 15;47:545–550. doi: 10.1073/pnas.47.4.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Karess R. E., Rubin G. M. A small tandem duplication is responsible for the unstable white-ivory mutation in Drosophila. Cell. 1982 Aug;30(1):63–69. doi: 10.1016/0092-8674(82)90012-5. [DOI] [PubMed] [Google Scholar]
  10. Kaufman T. C., Tasaka S. E., Suzuki D. T. The interaction of two complex loci, zeste and bithorax in Drosophila melanogaster. Genetics. 1973 Oct;75(2):299–321. doi: 10.1093/genetics/75.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lefevre G., Jr, Green M. M. Genetic duplication in the white-split interval of the X chromosome in Drosophila melanogaster. Chromosoma. 1972;36(4):391–412. doi: 10.1007/BF00336795. [DOI] [PubMed] [Google Scholar]
  12. Levis R., Collins M., Rubin G. M. FB elements are the common basis for the instability of the wDZL and wC Drosophila mutations. Cell. 1982 Sep;30(2):551–565. doi: 10.1016/0092-8674(82)90252-5. [DOI] [PubMed] [Google Scholar]
  13. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pirrotta V., Hadfield C., Pretorius G. H. Microdissection and cloning of the white locus and the 3B1-3C2 region of the Drosophila X chromosome. EMBO J. 1983;2(6):927–934. doi: 10.1002/j.1460-2075.1983.tb01523.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  16. Steller H., Pirrotta V. Regulated expression of genes injected into early Drosophila embryos. EMBO J. 1984 Jan;3(1):165–173. doi: 10.1002/j.1460-2075.1984.tb01778.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  18. Zachar Z., Bingham P. M. Regulation of white locus expression: the structure of mutant alleles at the white locus of Drosophila melanogaster. Cell. 1982 Sep;30(2):529–541. doi: 10.1016/0092-8674(82)90250-1. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES