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. 1984 Mar;3(3):645–650. doi: 10.1002/j.1460-2075.1984.tb01861.x

Hepatitis B virus antigens made in microbial cells immunise against viral infection.

K Murray, S A Bruce, A Hinnen, P Wingfield, P M van Erd, A de Reus, H Schellekens
PMCID: PMC557401  PMID: 6370689

Abstract

Chimpanzees have been vaccinated successfully against hepatitis B virus with preparations of the viral antigens made in microbial cells by genetic engineering methods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berquist K. R., Maynard J. E., Murphy B. L. Letter: Infectivity of serum containing HBsAg and antibody to e antigen. Lancet. 1976 May 8;1(7967):1026–1027. doi: 10.1016/s0140-6736(76)91908-5. [DOI] [PubMed] [Google Scholar]
  2. Burrell C. J., Mackay P., Greenaway P. J., Hofschneider P. H., Murray K. Expression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR322. Nature. 1979 May 3;279(5708):43–47. doi: 10.1038/279043a0. [DOI] [PubMed] [Google Scholar]
  3. Cameron C. H., Combridge B. S., Howell D. R., Barbara J. A. A sensitive immunoradiometric assay for the detection of hepatitis B surface antigen. J Virol Methods. 1980;1(6):311–323. doi: 10.1016/0166-0934(80)90048-8. [DOI] [PubMed] [Google Scholar]
  4. Charnay P., Pourcel C., Louise A., Fritsch A., Tiollais P. Cloning in Escherichia coli and physical structure of hepatitis B virion DNA. Proc Natl Acad Sci U S A. 1979 May;76(5):2222–2226. doi: 10.1073/pnas.76.5.2222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Christman J. K., Gerber M., Price P. M., Flordellis C., Edelman J., Acs G. Amplification of expression of hepatitis B surface antigen in 3T3 cells cotransfected with a dominant-acting gene and cloned viral DNA. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1815–1819. doi: 10.1073/pnas.79.6.1815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dubois M. F., Pourcel C., Rousset S., Chany C., Tiollais P. Excretion of hepatitis B surface antigen particles from mouse cells transformed with cloned viral DNA. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4549–4553. doi: 10.1073/pnas.77.8.4549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Galibert F., Mandart E., Fitoussi F., Tiollais P., Charnay P. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature. 1979 Oct 25;281(5733):646–650. doi: 10.1038/281646a0. [DOI] [PubMed] [Google Scholar]
  8. Gough N. M., Murray K. Expression of the hepatitis B virus surface, core and E antigen genes by stable rat and mouse cell lines. J Mol Biol. 1982 Nov 25;162(1):43–67. doi: 10.1016/0022-2836(82)90161-9. [DOI] [PubMed] [Google Scholar]
  9. Hitzeman R. A., Chen C. Y., Hagie F. E., Patzer E. J., Liu C. C., Estell D. A., Miller J. V., Yaffe A., Kleid D. G., Levinson A. D. Expression of hepatitis B virus surface antigen in yeast. Nucleic Acids Res. 1983 May 11;11(9):2745–2763. doi: 10.1093/nar/11.9.2745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaplan P. M., Greenman R. L., Gerin J. L., Purcell R. H., Robinson W. S. DNA polymerase associated with human hepatitis B antigen. J Virol. 1973 Nov;12(5):995–1005. doi: 10.1128/jvi.12.5.995-1005.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Levin J., Bang F. B. Clottable protein in Limulus; its localization and kinetics of its coagulation by endotoxin. Thromb Diath Haemorrh. 1968 Mar 31;19(1):186–197. [PubMed] [Google Scholar]
  12. MacKay P., Lees J., Murray K. The conversion of hepatitis B core antigen synthesized in E coli into e antigen. J Med Virol. 1981;8(4):237–243. doi: 10.1002/jmv.1890080404. [DOI] [PubMed] [Google Scholar]
  13. MacKay P., Pasek M., Magazin M., Kovacic R. T., Allet B., Stahl S., Gilbert W., Schaller H., Bruce S. A., Murray K. Production of immunologically active surface antigens of hepatitis B virus by Escherichia coli. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4510–4514. doi: 10.1073/pnas.78.7.4510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Magnius L. O., Espmark J. A. New specificities in Australia antigen positive sera distinct from the Le Bouvier determinants. J Immunol. 1972 Nov;109(5):1017–1021. [PubMed] [Google Scholar]
  15. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  17. Meyhack B., Bajwa W., Rudolph H., Hinnen A. Two yeast acid phosphatase structural genes are the result of a tandem duplication and show different degrees of homology in their promoter and coding sequences. EMBO J. 1982;1(6):675–680. doi: 10.1002/j.1460-2075.1982.tb01229.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moriarty A. M., Hoyer B. H., Shih J. W., Gerin J. L., Hamer D. H. Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2606–2610. doi: 10.1073/pnas.78.4.2606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Neurath A. R., Trepo C., Chen M., Prince A. M. Identification of additional antigenic sites on Dane particles and the tubular forms of hepatitis B surface antigen. J Gen Virol. 1976 Mar;30(3):277–285. doi: 10.1099/0022-1317-30-3-277. [DOI] [PubMed] [Google Scholar]
  20. Ohori H., Onodera S., Ishida N. Demonstration of hepatitis B e antigen (HBeAg) in association with intact Dane particles. J Gen Virol. 1979 May;43(2):423–427. doi: 10.1099/0022-1317-43-2-423. [DOI] [PubMed] [Google Scholar]
  21. Okada K., Kamiyama I., Inomata M., Imai M., Miyakawa Y. e antigen and anti-e in the serum of asymptomatic carrier mothers as indicators of positive and negative transmission of hepatitis B virus to their infants. N Engl J Med. 1976 Apr 1;294(14):746–749. doi: 10.1056/NEJM197604012941402. [DOI] [PubMed] [Google Scholar]
  22. Pasek M., Goto T., Gilbert W., Zink B., Schaller H., MacKay P., Leadbetter G., Murray K. Hepatitis B virus genes and their expression in E. coli. Nature. 1979 Dec 6;282(5739):575–579. doi: 10.1038/282575a0. [DOI] [PubMed] [Google Scholar]
  23. Robinson W. S. The genome of hepatitis B virus. Annu Rev Microbiol. 1977;31:357–377. doi: 10.1146/annurev.mi.31.100177.002041. [DOI] [PubMed] [Google Scholar]
  24. Shikata T., Karasawa T., Abe K., Uzawa T., Suzuki H., Oda T., Imai M., Mayumi M., Moritsugu Y. Hepatitis B e antigen and infectivity of hepatitis B virus. J Infect Dis. 1977 Oct;136(4):571–576. doi: 10.1093/infdis/136.4.571. [DOI] [PubMed] [Google Scholar]
  25. Sninsky J. J., Siddiqui A., Robinson W. S., Cohen S. N. Cloning and endonuclease mapping of the hepatitis B viral genome. Nature. 1979 May 24;279(5711):346–348. doi: 10.1038/279346a0. [DOI] [PubMed] [Google Scholar]
  26. Stahl S., MacKay P., Magazin M., Bruce S. A., Murray K. Hepatitis B virus core antigen: synthesis in Escherichia coli and application in diagnosis. Proc Natl Acad Sci U S A. 1982 Mar;79(5):1606–1610. doi: 10.1073/pnas.79.5.1606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Szmuness W., Stevens C. E., Harley E. J., Zang E. A., Oleszko W. R., William D. C., Sadovsky R., Morrison J. M., Kellner A. Hepatitis B vaccine: demonstration of efficacy in a controlled clinical trial in a high-risk population in the United States. N Engl J Med. 1980 Oct 9;303(15):833–841. doi: 10.1056/NEJM198010093031501. [DOI] [PubMed] [Google Scholar]
  28. Tabor E., Purcell R. H., London W. T., Gerety R. J. Use of and interpretation of results using inocula of hepatitis B virus with known infectivity titers. J Infect Dis. 1983 Mar;147(3):531–534. doi: 10.1093/infdis/147.3.531. [DOI] [PubMed] [Google Scholar]
  29. Tedder R. S., Bull F. G. Characterization of 'e' antigen associated with hepatitis B. Clin Exp Immunol. 1979 Mar;35(3):380–389. [PMC free article] [PubMed] [Google Scholar]
  30. Trepo C., Bird R. G., Zuckerman A. J. Correlations between the detection of e antigen or antibody and electron microscopic pattern of hepatitis B surface antigen (HBsAg) associated particles in the serum of HBsAg carriers. J Clin Pathol. 1977 Mar;30(3):216–220. doi: 10.1136/jcp.30.3.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Valenzuela P., Gray P., Quiroga M., Zaldivar J., Goodman H. M., Rutter W. J. Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen. Nature. 1979 Aug 30;280(5725):815–819. doi: 10.1038/280815a0. [DOI] [PubMed] [Google Scholar]
  32. Valenzuela P., Medina A., Rutter W. J., Ammerer G., Hall B. D. Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature. 1982 Jul 22;298(5872):347–350. doi: 10.1038/298347a0. [DOI] [PubMed] [Google Scholar]
  33. Werner B. G., Grady G. F. Accidental hepatitis-B-surface-antigen-positive inoculations. Use of e antigen to estimate infectivity. Ann Intern Med. 1982 Sep;97(3):367–369. doi: 10.7326/0003-4819-97-3-367. [DOI] [PubMed] [Google Scholar]
  34. Zanetti A. R., Ferroni P., Magliano E. M., Pirovano P., Lavarini C., Massaro A. L., Gavinelli R., Fabris C., Rizzetto M. Perinatal transmission of the hepatitis B virus and of the HBV-associated delta agent from mothers to offspring in northern Italy. J Med Virol. 1982;9(2):139–148. doi: 10.1002/jmv.1890090209. [DOI] [PubMed] [Google Scholar]

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