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. 1984 Apr;3(4):847–853. doi: 10.1002/j.1460-2075.1984.tb01895.x

Differential binding of a S. cerevisiae RNA polymerase III transcription factor to two promoter segments of a tRNA gene.

D J Stillman, E P Geiduschek
PMCID: PMC557437  PMID: 6233142

Abstract

A Saccharomyces cerevisiae protein fraction which binds specifically to the internal promoter regions of genes that are transcribed by RNA polymerase III is shown to function as a transcription factor. We postulate that the stable DNA binding of the factor confers stability on polymerase III transcription complexes. Analysis of the binding by DNase 'foot-printing' distinguishes three segments of the S. cerevisiae tRNALeu3 gene: a region surrounding the so-called A block of the internal promoter, a region surrounding the B block and an intermediate segment. Binding to the A and B block regions is connected, but the B block region exerts a dominant effect.

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Selected References

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