FIG 3 .

CsrA represses iraD mRNA and IraD protein levels. (A) qRT-PCR analysis of iraD mRNA levels in WT and csrA::kan mutant strains at mid-exponential phase. Values represent averages of results from 3 experiments ± standard deviations. (B) Western blot analysis of IraD levels in WT and csrA::gm strains grown to early (E), mid (M), and late (L) exponential phase. o/n, overnight cultures. Fold change values (csrA::gm/WT) are shown. Values are normalized to RpoB levels. Results are representative of two independent experiments.