Figure 6.

Androgen receptor RT-PCR. Intron-spanning primer sets for rat AR were used to amplify cDNA from young intact male rat substantia nigra pars compacta (SN), 2nd layer of the entorhinal cortex (ETC), CA1 region of the hippocampus (Hip), and N27 cells (A and B). Note the lack of amplification product for the 3′ region of AR in N27 cells (A). C, Targets of the antibodies used to detect AR and their epitopes in parentheses aligned with the AR domain structure (top), and the relative amplification product locations in the AR mRNA (bottom). Exons 2 and 3 code for the DBD. M denotes a 100-bp DNA ladder, and negative controls are marked with –.