Figure 4. Intracellular Ca²⁺ dependent calcineurin-NFAT signaling pathways regulate ACM redifferentiation.

A–B: The role of Ca²⁺ dependent calcineurin-NFAT signaling pathway in ACM redifferentiation. The percentage of ACMs with organized sarcomere (A) and spontaneous beating (B) were quantified at 7 days post-co-culture. The cells were pretreated with mouse specific siRNAs against calcineurin, NFATc3 and MEF2C, or calcineurin inhibitor CsA, respectively. N=6; * p<0.05 vs. Scramble; ^# p<0.05 vs. DMSO. C: Representative images of nuclear translocation of NFATc3 in ACMs at day 5 post-co-culture. The cells were pretreated with mouse specific siRNAs of Cx43 or scramble sequence as a control. Scale bars represent 20 μm.