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. 1984 Sep;3(9):2101–2106. doi: 10.1002/j.1460-2075.1984.tb02097.x

Sequence analysis of a split gene involved in fruiting from the fungus Schizophyllum commune

J J M Dons 1,*, G H Mulder 1, G J A Rouwendal 1, J Springer 1, W Bremer 1, J G H Wessels 1
PMCID: PMC557649  PMID: 16453552

Abstract

The sequence of a gene and its mRNA, which is abundantly expressed during fruiting body initiation in the Basidiomycete Schizophyllum commune, is described. This gene (1G2), the first to be analyzed in this group of fungi, contains an open reading frame coding for a polypeptide of 94 amino acids and a mol. wt. of 9842. A possible signal peptide of ˜20 residues and one glycosylation site were found. The sequence analysis was hampered by a sequence rearrangement in one of the cDNA clones, probably due to base pairing between short complementary sequences present at the 5' and 3' ends of the mRNA. The 5' untranslated leader sequence is 57 bp long and harbors a possible ribosome binding site close to the AUG start codon. A TATA box is found at position −31 upstream of transcription initiation. The 3' untranslated sequence is ˜200 bp long and contains the sequence -TATATAAT-, which most likely represents the polyadenylation signal. Some heterogeneity as to the site of addition of the poly(A) tail was observed. The coding region of the gene is interrupted by three very small introns of 53, 49 and 49 bp, respectively. The 5' and 3' splice junctions are conserved: GTGAGT- and -AG-, respectively. Each intron contains a sequence complementary to the 5' end of the intron. These sequences are compared with internal conserved sequences in yeast and filamentous fungi with regard to their possible role in splicing.

Keywords: cloning artefact, fungal gene, Schizophyllum commune, sequence analysis, splicing

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Selected References

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