Abstract
Sulfolobus acidocaldarius, strain B12, which harbours a double-stranded DNA species both as a plasmid and in a linear form, which is integrated at a specific site of the chromosome, produces virus-like particles upon u.v. irradiation. These particles contain the same circular DNA and a number of coat proteins and are probably surrounded by a lipid membrane. They are lemon shaped, 100 x 60 nm in size and carry tail structures at one pole. The host cell recovers and remains lysogenic after virus production. Though a large fraction of liberated particles is found attached to structures derived from the cells, neither adsorption nor infection of a number of Sulfolobus isolates has so far been observed.
Keywords: archaebacteria, integration, lipoproteins, plasmid, virus
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Espejo R. T., Canelo E. S., Sinsheimer R. L. DNA of bacteriophage PM2: a closed circular double-stranded molecule. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1164–1168. doi: 10.1073/pnas.63.4.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J. The phylogeny of prokaryotes. Science. 1980 Jul 25;209(4455):457–463. doi: 10.1126/science.6771870. [DOI] [PubMed] [Google Scholar]
- Schnabel H. An immune strain of Halobacterium halobium carries the invertible L segment of phage PhiH as a plasmid. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1017–1020. doi: 10.1073/pnas.81.4.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schnabel H., Zillig W., Pfäffle M., Schnabel R., Michel H., Delius H. Halobacterium halobium phage øH. EMBO J. 1982;1(1):87–92. doi: 10.1002/j.1460-2075.1982.tb01129.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tu J. K., Prangishvilli D., Huber H., Wildgruber G., Zillig W., Stetter K. O. Taxonomic relations between archaebacteria including 6 novel genera examined by cross hybridization of DNAs and 16S rRNAs. J Mol Evol. 1982;18(2):109–114. doi: 10.1007/BF01810829. [DOI] [PubMed] [Google Scholar]
- Tucker W. T., Pemberton J. M. Viral R plasmid Rphi6P: properties of the penicillinase plasmid prophage and the supercoiled, circular encapsidated genome. J Bacteriol. 1978 Jul;135(1):207–214. doi: 10.1128/jb.135.1.207-214.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wais A. C., Kon M., MacDonald R. E., Stollar B. D. Salt-dependent bacteriophage infecting Halobacterium cutirubrum and H. halobium. Nature. 1975 Jul 24;256(5515):314–315. doi: 10.1038/256314a0. [DOI] [PubMed] [Google Scholar]
- Woese C. R., Fox G. E. Phylogenetic structure of the prokaryotic domain: the primary kingdoms. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5088–5090. doi: 10.1073/pnas.74.11.5088. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yeats S., McWilliam P., Zillig W. A plasmid in the archaebacterium Sulfolobus acidocaldarius. EMBO J. 1982;1(9):1035–1038. doi: 10.1002/j.1460-2075.1982.tb01292.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zillig W., Stetter K. O., Janeković D. DNA-dependent RNA polymerase from the archaebacterium Sulfolobus acidocaldarius. Eur J Biochem. 1979 Jun 1;96(3):597–604. doi: 10.1111/j.1432-1033.1979.tb13074.x. [DOI] [PubMed] [Google Scholar]
- Zillig W., Stetter K. O., Tobien M. DNA-dependent RNA polymerase from Halobacterium halobium. Eur J Biochem. 1978 Nov 2;91(1):193–199. doi: 10.1111/j.1432-1033.1978.tb20951.x. [DOI] [PubMed] [Google Scholar]