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. 1984 Nov;3(11):2499–2504. doi: 10.1002/j.1460-2075.1984.tb02163.x

Cloning of heat-shock locus 93D from Drosophila melanogaster.

U Walldorf, S Richter, R P Ryseck, H Steller, J E Edström, E K Bautz, B Hovemann
PMCID: PMC557719  PMID: 6096125

Abstract

Using the microcloning approach a number of recombinant lambda phages carrying DNA from the 93D region have been isolated. Screening genomic libraries, cloned in phage lambda or cosmid vectors, with this isolated DNA yielded a series of overlapping DNA fragments from the region 93D6-7 as shown by in situ hybridization to polytene chromosomes. In vitro 32P-labelled nuclear RNA prepared from heat-shocked third instar larvae hybridized specifically to one fragment within 85 kb of cloned DNA. The region which is specifically transcribed after heat shock could be defined to a cluster of internally-repetitive DNA and its neighbouring proximal sequences. Over a sequence of 10-12 kb in length the DNA is cut into repeat units of approximately 280 nucleotides by the restriction endonuclease TaqI. The TaqI repeat sequences are unique in the Drosophila genome.

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Selected References

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