Table 3.
Amino acid (aa) substitutions detected at baseline by Sanger sequencing in non-structural protein 5A (NS5A) and non-structural protein 5B (NS5B) from 42 patients for whom sequence data are available. Substitutions that differed from the reference sequence (D17763) are reported. The numbers in subscript text next to the amino acid substitution represent the frequency of each polymorphism. Substitutions known to reduce susceptibility to DAAs in HCV GT3a patients are shown in bold.
| Non-Structural Protein 5A (NS5A) | ||||||||||||||||
| Reference | D40.5 | D92.9 | R95.2 | T52.4 | D97.6 | S83.3 | L100.0 | A4.8 | K97.6 | A9.5 | L97.6 | K97.6 | M97.6 | A90.5 | L97.6 | I97.6 |
| aa | 2 | 3 | 6 | 7 | 10 | 14 | 16 | 17 | 20 | 21 | 23 | 26 | 28 | 30 | 34 | 37 |
| Variants % | G61.9 | E7.1 | H4.8 | A2.4 | E2.4 | I2.4 | V2.4 | S90.5 | R2.4 | T92.9 | I2.4 | R2.4 | L2.4 | K9.5 | I2.4 | L2.4 |
| N2.4 | D23.8 | L2.4 | T2.4 | |||||||||||||
| I14.3 | T16.7 | Y2.4 | ||||||||||||||
| V16.7 | ||||||||||||||||
| Reference | K95.2 | Y97.6 | K97.6 | V97.6 | M100.0 | S97.6 | T97.6 | P92.9 | A2.4 | I97.6 | T88.1 | V100.0 | L97.6 | A92.9 | P97.6 | T90.5 |
| aa | 41 | 43 | 44 | 46 | 53 | 54 | 55 | 58 | 62 | 63 | 64 | 67 | 74 | 75 | 77 | 79 |
| Variants % | R4.8 | F2.4 | R2.4 | A2.4 | V2.4 | A2.4 | A2.4 | A2.4 | P2.4 | L2.4 | A7.1 | I2.4 | I2.4 | V9.5 | S2.4 | A2.4 |
| L2.4 | S2.4 | S85.7 | S4.8 | M2.4 | M2.4 | |||||||||||
| T2.4 | T14.3 | R2.4 | ||||||||||||||
| S2.4 | ||||||||||||||||
| Reference | M97.6 | H73.8 | Y95.2 | S66.7 | S54.8 | P97.6 | T83.3 | W97.6 | N90.5 | S97.6 | ||||||
| aa | 83 | 85 | 93 | 98 | 103 | 104 | 107 | 111 | 116 | 117 | ||||||
| Variants % | T4.8 | C2.4 | D4.8 | G35.7 | A4.8 | L2.4 | A2.4 | L2.4 | S9.5 | G2.4 | ||||||
| R2.4 | H2.4 | P45.2 | K2.4 | |||||||||||||
| Y28.6 | N2.4 | N2.4 | ||||||||||||||
| S7.1 | ||||||||||||||||
| Non-Structural Protein 5B (NS5B) | ||||||||||||||||
| Reference | P95.2 | G100.0 | V100 | Q97.6 | I90.5 | E88.1 | T95.2 | G97.6 | P31.0 | K81.0 | S95.2 | T92.9 | Q97.6 | V95.2 | I97.6 | N90.5 |
| aa | 156 | 166 | 169 | 180 | 184 | 185 | 186 | 188 | 189 | 206 | 210 | 213 | 231 | 235 | 239 | 244 |
| Variants % | A4.8 | R2.4 | I2.4 | K2.4 | L4.8 | A11.9 | A2.4 | S2.4 | A4.8 | E2.4 | A4.8 | A4.8 | R2.4 | G2.4 | V2.4 | D11.9 |
| T4.8 | G2.4 | V2.4 | S64.3 | Q14.3 | N2.4 | M4.8 | ||||||||||
| T2.4 | ||||||||||||||||
| Reference | E97.6 | R90.5 | S97.6 | S97.6 | F97.6 | K97.6 | A85.7 | Q95.2 | C100.0 | I95.2 | T100.0 | K76.2 | N0.0 | R97.6 | N97.6 | F97.6 |
| aa | 246 | 250 | 254 | 255 | 267 | 270 | 272 | 273 | 274 | 293 | 300 | 304 | 307 | 309 | 310 | 313 |
| Variants % | Q2.4 | K11.9 | T2.4 | A2.4 | Y2.4 | R2.4 | D16.7 | P4.8 | W2.4 | L4.8 | S2.4 | R26.2 | G97.6 | Q2.4 | D4.8 | L2.4 |
| S2.4 | ||||||||||||||||