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. 2017 Aug 22;89(8):762–770. doi: 10.1212/WNL.0000000000004262

Figure 1. Location and protein expression levels of human GNAO1 mutations related to epileptic encephalopathy.

Figure 1

(A) Location of 15 mutations (G40R, G42R, D174G, T191_F197del, L199P, G203R, R209C, R209G, R209H, A227V, Y231C, E246K, N270H, F275S, and I279N) mapped on the Gαo amino acid sequence. (B, C) Representative Western blots of Gαo protein expression from HEK293T cells transiently transfected with each Gαo mutant. (D, E) Quantification of relative protein levels of each Gαo mutant compared to wild-type Gαo. Graphs are the result of 3 independent experiments and data are presented as mean ± SEM. **p < 0.01, ****p < 0.0001 using 1-way analysis of variance with Bonferroni post hoc test for pairwise comparison.