Map for ROS1
(A) and RET
(B) dual color break-apart probes (ZytoLight® SPEC ROS1 and RET, ZytoVision). For both genes, the orange and green fluorochrome direct labeled probes hybridize proximal (5′ end) and distal (3′ end) to the genes, respectively. The known breakpoints in ROS1 are located in introns 31, 33 and 34 and are proximal to the kinase domain (exons 36 to 41). For RET, the known breakpoints are located in introns 10 and 11 and are proximal to the kinase domain (exons 12 to 19). The precise location of the genes on the chromosome, the size (kb) and orientation of the genes are indicated according to the Human assembly GRCh37/hg19. kb: kilobase pair; Cen: centromere; Tel: telomere; Chr: chromosome; KD: kinase domain.