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. 2017 Jun 27;8(32):53581–53601. doi: 10.18632/oncotarget.18655

Figure 8. lncRNA H19 mediates BMP9-induced osteogenic differentiation by modulating the miRNAs that target Notch signaling.

Figure 8

(A) BMP9-regulated expression of Notch-targeting miRNAs in MSCs. Subconfluent iMEFs were infected with AdBMP9 or AdGFP for 36h. Total RNA was isolated from the infected iMEFs and subjected to TqPCR analysis of the selected 17 miRNAs that target the Notch pathway based on TargetScan. The relative normalized expression of these miRNAs was subjected to clustering analysis using the Multiple Experiment Viewer (MeV). The eight miRNAs that were significantly differentially expressed are highlighted in red (a). The Notch receptors/ligands and the putative target sites for the selected miRNAs are shown (b). (B) The basal expression levels of the eight miRNAs in iMEF-SEB, iMEF-simH19 and iMEF-H19 cells. Total RNA was isolated from the subconfluent cells and subjected to TqPCR. Gapdh was used as a reference gene. “*” p < 0.05, “**” p < 0.001, compared with that of the iMEF-SEB group. (C) The effect of BMP9 on the expression of miRNAs in iMEF-simH19 cells. The cells were infected with AdBMP9 or AdGFP for 36h. Total RNA was isolated and subjected to TqPCR analysis. Gapdh was used as a reference gene. “*” p < 0.05, “**” p < 0.001, compared with that of the AdGFP group. (D) The effect of BMP9 on the expression of miRNAs in iMEF-H19 cells. The cells were infected with AdBMP9 or AdGFP for 36h. Total RNA was isolated and subjected to TqPCR analysis. Gapdh was used as a reference gene. “*” p<0.05, “**” p<0.001, compared with that of the AdGFP group.