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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: Methods. 2017 Jun 1;126:112–129. doi: 10.1016/j.ymeth.2017.05.028

Figure 4. Hotter reverse transcription reduces untemplated extension without reducing yield.

Figure 4

Reverse transcription of ligated footprints (left), linker only (middle), or RT primer alone (right) with Protoscript II at temperatures ranging from 46°C – 57°C. Reaction were resolved by polyacrylamide gel electrophoresis as described in Section 5.3. An example of non-templated addition to the reverse transcription (RT) primer is indicated. L – 10 bp ladder, the 100 nt band and selected other bands are indicated on the left-hand side of the gel.