Figure 6. Library construction PCR gel.

As outlined in Section 5.9, each sample is loaded across two lanes of the gel. Note the downwards smear in lanes 4 & 5 that is absent from lanes 2 & 3, below the pink box denoting the area of the gel that was excised for purification of the full-length library. This smear likely represents no-insert product and unelongated reverse transcription primer that has persisted through the cDNA size-selection gel. The size (nucleotides)of several bands from the 10 bp ladder are indicated on the left-hand side of the gel.