Table 16.

Composition of the library construction PCR reaction

Component	Amount per run (µl)	Final
5X Phusion HF buffer	10	1X
dNTPs 10 mM ea.	1	0.2 mM ea.
10 µM Forward Primer	2.5	500 nM
10 µM Reverse Primer	2.5	500 nM
Circularized cDNA template	see above	1 – 256 pM
Nuclease free water	q.s. to 49.5 µl	NA
Phusion polymerase (2 U/µl)	0.5	1 U