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. 2017 May 25;8(31):51591–51607. doi: 10.18632/oncotarget.18196

Figure 7. Vascular co-option between human pericytes and rat endothelial cells.

Figure 7

(A) Validation of human chromosome painting FISH probe in green, rodent probe in red, and DAPI in blue using human U-251 and rat GH3 cells. (B-C) FISH analysis of rat endothelial cell layers in red (arrows) enclosed by human tumor cells. Non-specific signals are in white due to the overlay of red and green. Boxed area of an enlarged vessel shows disintegrated rat endothelial layer in red enclosed by human cells in green. (D) Immunohistochemical staining of human specific HLA-A, with boxed areas enlarged showing rat capillaries captured by human pericytes. Arrows indicate pericytes. (E) Immunohistochemistry and FISH analyses using adjacent sections. GT198+ pericytes (black arrows) are HLA-A+ and VEGF+, and enclose rat endothelial cells (white arrows). Scale bars = 100 μm.