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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Virology. 2017 Mar 27;506:84–91. doi: 10.1016/j.virol.2017.03.011

Figure 5. Expansion of wild-type and V163 mutant Prohead II.

Figure 5

Proheads were treated with DMF expansion buffer for the indicated times and analyzed on a 0.8% agarose gel. The positions of Prohead II and expanded Heads are indicated in the figure. The V163D mutant particle positions are indicated by thicker lines on the left. The mutant proheads migrate faster than wild-type proheads, as also seen in Figure 4(a), presumably because of the increased negative charge from the V163D mutation. This added negative charge is apparently buried during expansion, so the mutant heads no longer migrate faster than wild type.