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. 2017 Jun 8;13(8):1318–1330. doi: 10.1080/15548627.2017.1327103

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Gcn5 represses light-induced autophagy. (A) RFP-Atg8 in dark- or light- cultured mycelia of the WT, gcn5Δ and GCN5OX strains. Scale bar: 5 μm. (B) Total protein lysates from the indicated strains were analyzed by immunoblotting with anti-RFP antibodies, under light or dark conditions. The extent of autophagy was estimated by calculating the amount of free RFP compared with the total amount of intact RFP-Atg8 and free RFP (the numbers appear underneath the blot). Densitometric analysis was performed using ImageJ (https://imagej.nih.gov/ij/). (C) GFP-Gcn5 signal in the GCN5OX strain appears nuclear (arrowhead) as well as cytosolic (arrow). Scale bar: 5 μm. DIC, differential interference contrast.