Skip to main content
. 2017 Jul 26;207(1):115–128. doi: 10.1534/genetics.117.300057

Figure 2.

Figure 2

Detection of DSB-induced reciprocal COs. Red and black lines indicate replicated sister chromatids of the W303 and YJM chromosome IV homologs, respectively; ● corresponds to centromeres. (A) Red–white sectors correspond to reciprocal CO events. The starting diploid is pink because it contains a single copy of SUP4-o (▿) and suppression of the ade2-1 alleles is incomplete. A CO followed by segregation of sister chromatids into different daughter cells yields a cell with no copy of SUP4-o and a cell with two copies. The former and latter produce the red and white sides of sectored colony. If only one of the W303/red sister chromatids is broken (left side), the transfer of information from a donor YJM/black chromatid produces a 3:1 GC tract. If both red chromatids are broken, a hybrid 4:0/3:1 tract is produced. (B) Sequence inserted into the W303 and YJM chromosomes. Only the W303 chromosome is cleaved by I-SceI.