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. 2017 Sep 5;8(5):e00940-17. doi: 10.1128/mBio.00940-17

FIG 2 .

FIG 2 

Impact of ectopic expression of SID-3 in sid-3(vir9) mutant on Orsay virus replication and GFP expression. (A) Neighbor-joining phylogenetic tree of SID-3 orthologs from humans (Homo sapiens) and multiple model organisms. The model organisms include Gallus gallus, Danio rerio, Xenopus tropicalis, Drosophila melanogaster, Saccharomyces pombe, and Mus musculus. The bar indicates the amino acid substitution rate (0.1 amino acid substitution per position). (B) Schematic representation of protein domain organization of C. elegans SID-3 and human TNK2. (C) Imaging of Orsay virus-infected sid-3(vir9) mutant that overexpressed wild-type SID-3 or the K139A kinase catalytic domain mutant. BF, bright-field microscopy. Bars, 200 μm. (D) Real-time qRT-PCR of Orsay virus-infected sid-3(vir9) mutant that overexpressed wild-type SID-3 or the K139A kinase catalytic domain mutant. **, P < 0.01; NS, not significant (P > 0.05).