Skip to main content
. Author manuscript; available in PMC: 2017 Sep 7.
Published in final edited form as: Structure. 2014 Nov 13;22(12):1754–1763. doi: 10.1016/j.str.2014.09.012

Figure 5.

Figure 5

Time-dependent oxidation of X1NTD by H2O2. The fraction of oxidized X1NTD, fox, determined from the Val72 methyl resonance using Equation 1, is plotted as a function of time after the addition of H2O2. The traces correspond to: A) wt X1NTD (TC = 7.6 h); B) X1NTD(H8Y) (TC = 5.9 h); C) X1NTD plus 100 nM CuCl2 (TC = 3.0 h); D) X1NTD plus 5.8 µM PDI (TC = 1.9 h). For the sample shown in panel C, the initial fiox = 0.36, for the remaining samples, fiox < 0.1. The mean time constants are reported in Table 1. All samples contained 25 µM X1NTD in 50 mM Tris-d11, pH 7.6, 140 mM NaCl, 0.5 mM EDTA, 0.25 mM NaN3, and 75 mM bicarbonate. Experiments were run at 30 °C on a Varian INOVA 600.