Figure 2.

Mammalian target of rapamycin complex 1 (mTORC1) supported plasma cell differentiation and humoral response against acute lymphocytic choriomeningitis virus (LCMV) infection. (a) Flow cytometry of IgD⁻ lymphocytes (top left), CD138⁺ B220^mi/lo plasma cell total number (top right), and quantification of Prdm1 in plasma cells from wild‐type (WT) and Rptor‐KO mice on day 12 after LCMV infection. (b) Quantification of LCMV‐specific IgG in serum of Rptor‐KO and WT mice after the indicated post‐infection time (top). The concentrations were normalized to that of WT mice on post‐infection day 8. Quantification of LCMV titres in spleens and serum of Rptor‐KO and WT mice on post‐infection day 8 (bottom). (c) Flow cytometry of B220⁺ B cells and quantification of 4‐hydroxy‐3‐nitrophenylacetyl (NP)‐specific GCB (NP ⁺ Ig‐λ ⁺ PNA ⁺ CD95⁺ B220⁺) cells and NP‐specific plasma cells (NP ⁺ Ig‐λ ⁺ CD138⁺ B220^mi/lo) among total cell number in spleens from wild‐type B1‐8^hi (WT B1‐8) and Aicda‐Cre‐Rptor ^flox B1‐8^hi (Rptor‐KO B1‐8) mice on day 8 after LCMV infection. *P < 0·05 **P < 0·005 ***P < 0·002 (unpaired two‐tailed t‐test). Data are representative of three independent experiments with three to six mice per group (error bars, SEM). [Colour figure can be viewed at wileyonlinelibrary.com]