Figure 4.

The cell cycle phase distribution in wt and p21^−/− HCT116 cells treated with SCH900776 and LA-12/cisplatin. Percentage of wt (A and B) and p21^−/− (C and D) HCT116 cells in individual cell cycle phases (flow cytometry) following their pretreatment (2 hours) with SCH900776 (SCH, 3 μM) and subsequent 48-hour (A and C) or 72-hour (B and D) treatment with LA-12 (0.75 μM) or cisplatin (CDDP, 20 μM). Results are means ± SEM of three independent experiments. Statistical significance: P < .05, * versus control, ‡ versus SCH and Ο versus LA-12 or CDDP.