Table 4. Reproducibility of the LAMP testing using clinical and cultured samples.

Samples	Runa	Time to amplify (min)	Melt (°C)	Time (Mean + SD)	Melt (Mean + SD)
C. pecorumpositive samples
Koala rectal swab	1	20.15	83.44	20.53, 0.54	83.32, 0.16
	2	20.30	83.37
	3	21.15	83.14
Marsbar DNA	1	13.50	83.50	13.27, 0.20	83.55, 0.06
	2	13.15	83.52
	3	13.15	83.62
Koala A2 DNA	1	12.00	83.35	11.43, 0.51	83.41, 0.05
	2	11.00	83.45
	3	11.30	83.43
RI koala UGT swab	1	17.00	83.34	17.72, 0.62	83.21, 0.12
	2	18.00	83.21
	3	18.15	83.09
L14 DNA	1	13.15	83.53	13.15, 0	83.50, 0.02
	2	13.15	83.50
	3	13.15	83.48
HsLuRz DNA	1	13.45	83.49	13.63, 0.32	83.40, 0.08
	2	13.45	83.36
	3	14.00	83.34
K20 cloaca swab	1	22.00	82.83	22.2, 0.23	83.01, 0.19
	2	22.15	83.00
	3	22.45	83.20
C. psittacipositive samples
Cr009 DNA	1	6.45	84.30	6.40, 0.09	84.33, 0.03
	2	6.45	84.36
	3	6.30	84.34
HoRE DNA	1	5.00	84.46	5.10, 0.08	84.45, 0.14
	2	5.15	84.58
	3	5.15	84.30
B2 DNA	1	10.30	84.08	10.10, 0.17	84.17, 0.08
	2	10.00	84.20
	3	10.00	84.24
Horse placental swab	1	11.15	82.90	10.58, 0.49	83.19, 0.26
	2	10.30	83.42
	3	10.30	83.24
Horse_pl DNA	1	10.30	84.53	10.87, 0.98	84.41, 0.18
	2	12.00	84.21
	3	10.30	84.50

Notes.

^aThe assay was performed in Genie III Real-time fluorometer, with the amplification times and annealing temperatures recorded at the end of each run. The samples were tested in three different runs by two different operators.