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. 2017 Aug 3;8(34):55863–55876. doi: 10.18632/oncotarget.19866

Figure 7. Expression of the isolated iSH2 domains enhances oncogenic and signaling activity of PI3K.

Figure 7

A. Representative focus assays of p85α, p85β, iSH2α and iSH2β. Both iSH2 domains induce a thickening of the cell sheet that is indicative of oncogenic transformation. The effect is not as pronounced as that induced by p85β, but is evident in comparison with the non-transforming p85α. B. Western blots documenting enhanced PI3K signaling with pAKT S473 and pS6. Expression of the iSH2α domain with the HA tag was not clearly demonstrable in standard Western blots and was therefore documented with qRTPCR C. The positive controls in this figure represent standard PCR from the DNA constructs; the HA iSH2α and iSH2β bands were generated by qRTPCR.