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. 2017 Apr 28;8(34):56582–56597. doi: 10.18632/oncotarget.17508

Figure 4. Doxorubicin exposure induces DRP1 accumulation in mitochondria.

Figure 4

(A) Doxorubicin (DOX) upregulated dynamic-related protein 1 (DRP1) expression in whole cell lysate. AGS cells were treated with 1μmol/L DOX and then harvested at the indicated time for immunoblot analysis of DRP1. (B) and (C) DRP1 translocates from cytosol to mitochondria, whereas cytochrome-c (Cyt-c) releases from mitochondria to cytosol upon DOX exposure. AGS cells were treated with 1μmol/L DOX and then harvested at the indicated time for immunoblot analysis of DRP1 and Cyt-c expression in the mitochondria and cytosol. (A-C) Left panels show immunoblot. (A-C) Right panels show densitometry analysis. HM= mitochondria-enriched heavy membranes. Cytochrome c oxidase (COXIV) served as a loading control for HM and β-actin served as a loading control for whole cell lysate and cytosolic fraction. Figures presented are the representative of at least three independent experiments. The densitometry data were expressed as the mean±SEM of three independent experiments. *P<0.05, ***P<0.001, and ****P<0.0001 versus non-treated control.