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. 2017 Jun 16;8(34):57012–57023. doi: 10.18632/oncotarget.18505

Figure 5. Knockdown of EGFR inhibits cell proliferation, cell cycle and promote apoptosis in HCC827 cell lines.

Figure 5

(A) Relative expression of EGFR in NSCLC and corresponding non-tumor tissues (n = 61). (B) Expression of EGFR in the human NSCLC cell lines and a BEAS-2B was measured by qRT-PCR. (C) EGFR mRNA was detected by qRT-PCR in HCC827 cells transfected with EGFR siRNA or siNC. (D) The protein levels of EGFR were detected by western blot in HCC827 cells transfected with EGFR siRNA or siNC. (E) The proliferation of HCC827 cells transfected with EGFR siRNA or siNC, as determined by CCK-8 assay. (F) Cell cycle distribution was analyzed by flow cytometry in HCC827 cells transfected with EGFR siRNA or siNC. (G) Cell apoptosis distribution was analyzed by flow cytometry in HCC827 cells transfected with EGFR siRNA or siNC. Each experiment was performed in triplicate at least (*P < 0.05, **P < 0.01, ***P < 0.001).