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. 2017 Jul 18;8(34):57216–57230. doi: 10.18632/oncotarget.19332

Figure 6. K75/K125 acetylation of AuA promotes cell migration.

Figure 6

(A) Acetylation of AuA promotes cell migration. Wound healing assay of MCF7 cells was performed, the closure ratios were analyzed after 24 h.*p < 0.05. Error bar indicates S.E.M (n = 3). Scale bar, 100 μm. (B) Acetylation of AuA promotes cell invasion. Cells were seeded in inserted transwell. After 24 h, transwell was stained with crystal violet and the invasive cells were counted under microscope. *p < 0.05. Error bar indicates S.E.M (n = 3). Scale bar, 50 μm. (C) Acetylation of AuA is upregulated in migrating cell. Cells were scratched to activate cell migration and the lysates were immunoprecipitated with anti-Lys-Ac antibody or p-Ser/Thr and immunoblotting using anti-AuA antibody. (D) Acetylation of AuA activates AKT by p38 signaling but not ERK or FAK. Expression level of target proteins were analyzed by western blot and quantified by Image J program. Vinculin was used as loading control. *p < 0.05; **p < 0.005. Error bar indicates S.E.M (n = 3).