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. 2017 Jun 5;6(8):e1336272. doi: 10.1080/2162402X.2017.1336272

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© 2017 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 1. — HCMV positivity in GBM determined comparatively by serology, qPCR and IHC. GBM patients were investigated for presence of HCMV pp65 and IE-1 expression by (A) qPCR, IHC and serology (dark gray bars = positive samples and light gray bar = negative samples) and (B) IE-1 expression on macrophage/myeloid derived cells (left panel) and on tumor cells (right panel). Magnification 400X, Scale bar 100 μm. (C) Venn diagram showing comparative HCMV serology (IgG), qPCR (pp65), qPCR (IE-1) and IHC (IE-1) detection in GBM tissue and blood. (D) Venn diagram showing comparative HCMV pp65 and IE-1 qPCR in GBM patients' blood and tumor. (E) Representative dotplots showing (left to right) macrophages (CD45^brightCD11b^bright) and microglia (CD45^dimCD11b^bright) within tumor biopsies cells; pp65 vs. CD45 within macrophages of tumor biopsies; pp65 vs. CD31 within CD45⁻ tumor biopsy cells; and pp65 vs. CD45 within CD45⁻ tumor biopsy cells. (F) % mean ± SEM of pp65⁺ cells within CD45^brightCD11b^bright macrophages, CD45^dimCD11b^bright microglia, CD45-CD31+ endothelial cells and CD45- tumor cells. (2-way ANOVA, Bonferroni's multiple comparison, ****P < 0.0001). IHC = immunonohistochemistry; IE-1 = immediate early -1.