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. 2017 Jul 23;7(13):3293–3305. doi: 10.7150/thno.19988

Figure 5.

Figure 5

Inhibition of the SCD1 and FADS2 metabolic genes promotes ferroptosis. (A, B) RT-qPCR analysis was conducted to detect SCD1 (A) and FADS2 (B) in A549 cells that were stably transfected with two distinct target gene shRNA expression vectors and in control cells (siCTRL). (C, D) The MTT assay was performed to assess A549 cell viability after stable depletions of SCD1 (C) or FADS2 (D). (E-H) The Total iron and ferrous iron levels were analyzed in A549 cells in the depletion of SCD1 (E, F) and FADS2 (G, H). (I-L) Lipid ROS was measured by C11-BODIPY staining coupled to flow cytometry in A549 cells after the depletion of SCD1 (I) and FADS2 (J). A549 cells responses to erastin (5μM) ± Ferrostatin(1μM) after depletion of SCD1 (K) and FADS2 (L). * P<0.05, ** P<0.01, *** P<0.001.