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. 2017 Jun 30;24(10):1705–1716. doi: 10.1038/cdd.2017.94

PD-L1 and IAPs co-operate to protect tumors from cytotoxic lymphocyte-derived TNF

Conor J Kearney 1,2,5,*, Najoua Lalaoui 3,4, Andrew J Freeman 1, Kelly M Ramsbottom 1, John Silke 3,4, Jane Oliaro 1,2,5,*
PMCID: PMC5596429  PMID: 28665401

Abstract

Smac-mimetics are emerging as promising anti-cancer agents and are being evaluated in clinical trials for a variety of malignancies. Smac-mimetics can induce TNF production from a subset of tumor cells and simultaneously sensitize them to TNF-induced apoptosis. However, TNF derived from other cellular sources, such as cytotoxic lymphocytes (CLs) within the tumor, may also contribute to the anti-tumor activity of SMs. Here, we show that CD8+ T cells and NK cells potently kill tumor cells in the presence of the SM, birinapant. Enhanced CL killing occurred through TNF secretion upon tumor antigen recognition or NK-activating receptor ligation. Importantly, the perforin/granzyme route to CL-mediated tumor cell killing was dispensable for the efficacy of birinapant, emphasizing the importance of the TNF-mediated apoptosis pathway. Time-lapse microscopy revealed that birinapant sensitized tumor cells to apoptosis as bystanders and to membrane-bound TNF delivered to tumor cells within the immunological synapse. Furthermore, PD-L1 expression on tumor cells suppressed antigen-driven TNF production by CD8+ T cells, which could be antagonized through PD-1 blockade. Importantly, the elevated levels of TNF produced upon PD-1 blockade further enhanced tumor cell killing when combined with birinapant. The combined anti-tumor activity of IAP antagonism and PD-1 blockade occurred independently of perforin-mediated tumor cell death. Taken together, we identify CL-derived TNF as a potent effector of birinapant mediated anti-tumor immunity and opportunity for combination therapy through co-inhibition of immune checkpoints.

Inhibitor of apoptosis (IAP) proteins regulate diverse cellular functions including mitosis, migration, apoptosis, and signal transduction events that promote inflammation.1, 2 IAPs are defined by the presence of Baculoviral IAP Repeat (BIR) domains that facilitate their interaction with caspases to suppress apoptosis. Endogenous and synthetic IAP antagonists, such as Smac/Diablo and Smac-mimetics (SMs), respectively, can promote apoptosis upon interaction with IAP BIR domains.2, 3 Several IAP family members, such as the cellular IAPs (cIAP1/2) and XIAP, also possess a RING domain with E3 ubiquitin ligase activity, facilitating their involvement in signal transduction pathways.1, 2 Although XIAP can directly inhibit effector caspase protease activity, cIAP1/2 do not.4 Instead, cIAP1/2 restrain apoptosis induced by members of the TNF superfamily, including TNF itself, by initiating pro-survival canonical nuclear factor κB (NF-κB) activation and inhibiting the formation of death signaling complexes such as the Ripoptosome. As a result, several groups have shown that SM can sensitize tumor cells toward TNF-dependent apoptosis.5, 6, 7, 8 Indeed, several pharmaceutical companies have developed SMs and their clinical potential as cancer therapies, either as single agents, or in combination with other cytotoxic agents is being evaluated.

SMs display single agent efficacy against some tumors and leukemias.9, 10 This occurs via SM-induced TNF secretion, which acts in an autocrine fashion to kill the tumor cells in the absence of the cIAP1/2-mediated survival pathway.6, 7, 11 However, TNF or other cytokines, supplied by immune cells are likely to contribute to SM efficacy, especially in tumors that fail to produce TNF upon IAP loss.12, 13, 14, 15, 16, 17 In support of this concept, SMs are more efficient in vivo when combined with inflammatory adjuvants, which promote immune cell-derived TNF secretion.15, 18 Similarly, IAP antagonism promotes anti-tumor immunity in myeloma, independently from cell death induction.14 Therefore, although the mechanism by which SMs function in vivo is more complex than initially appreciated, combination therapies that boost immunity, particularly in the context of elevated TNF secretion, are likely to synergize with SMs.

Cytotoxic lymphocytes (CLs), including CD8+ T cells and natural killer (NK) cells are required for anti-tumor immunity and contribute to the efficacy of many cancer therapeutics.19, 20 CD8+ T-cell infiltration into diverse tumor types directly predicts patient prognosis.21, 22, 23 Similarly, proper NK cell function is required for tumor immune-surveillance,24 and is usually impaired in late-stage hematopoietic malignancy.25, 26 CLs clear tumors through a combination of direct perforin/granzyme-mediated cytotoxicity, amplifying anti-tumor immune responses via secretion of inflammatory cytokines such as TNF and through IFNγ-mediated tumor cytostasis.27, 28, 29 However, CLs often adopt an ‘exhausted’ phenotype within the tumor micro-environment, characterized by decreased effector function (cytotoxicity and cytokine production) and elevated expression of the inhibitory receptor PD-1.30, 31, 32 T-cell-driven immune responses are finely tuned by immune checkpoint receptors, including the PD-1/PD-L1 axis that serves to downregulate effector function and prevent excessive inflammation during infection.33, 34, 35 It is now clear however, that many tumors upregulate PD-L1 expression as a mechanism to antagonize CL-mediated anti-tumor immunity.32 Indeed, immune checkpoint blockade therapies have been successfully used in the clinic through reversing CD8+ T-cell exhaustion and re-instating effector function.30, 32, 36 However, whereas many studies have focused on the ability of checkpoint blockade therapies to enhance the activation state of CD8+ T cells, few studies have considered the anti-tumor effects of elevated TNF secretion upon immune checkpoint blockade.

A number of SMs, including birinapant, are currently in phase I/II clinical trials for a variety of cancer types (https://clinicaltrials.gov). Because SMs function in a TNF-dependent manner, and PD-1/PDL-1 blockade can enhance TNF secretion by CD8+ T cells,36 we examined if birinapant could enhance the anti-tumor activity of CLs.

Results

CLs produce inflammatory cytokines upon tumor cell recognition

Effector CD8+ T cells recognize tumor cells through the specific interaction between the T-cell receptor (TCR) and tumor antigens presented on MHC-I molecules. TCR engagement by its cognate antigen within the immunological synapse triggers signaling cascades that promote polarization of the microtubule organizing centre (MTOC) and lytic granules to the synaptic cleft to drive cell death via the perforin/granzyme pathway37, 38 and NF-κB-dependent production of inflammatory cytokines.37, 38 OT-I TCR transgenic CD8+ T cells efficiently lyse MC38 tumor cells stably expressing the OT-I antigen, ovalbumin (Ova, Figure 1a) and secrete abundant amounts of the inflammatory cytokines, TNF and IFNγ, (Figure 1b). CD8+ T cells formed an efficient immunological synapse with tumor cells in the presence (MC38-Ova), but not absence, of Ova (MC38-Vec), as evident from translocation of the MTOC toward the CL-tumor contact site (Figure 1c, quantitated in 1d). Localization of TNF and IFNγ at the synapse was observed in T-cell-tumor cell conjugates, and in bystander T cells (Figure 1e). These data demonstrate that effector CD8+ T cells continue to express abundant levels of TNF and IFNγ, even after completion of tumor cell lysis.

Figure 1.

Figure 1

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Cytotoxic lymphocytes produce inflammatory cytokines upon tumor cell recognition. (a) Chromium release assay (4 h) using MC38-Ova cells as targets and OT-I T cells as effectors. (b) MC38-Ova cells were exposed to OT-I T cells at the indicated effector to target (E:T) ratio. After 5 h, cytokines in supernatants were measured by CBA. (c) MC38-Ova or vector cells were seeded in chamber slides then overlaid with OT-I T cells. After 2 h, cells were fixed, stained as indicated, then visualized by confocal microscopy. (d) The percentage of T cells that were in a synapse with a target cell was quantitated by confocal microscopy. (e) The percentage of T cells that were positive for the indicated cytokines was quantitated by confocal microscopy. A minimum of 100 cells was counted in each condition. (f) Chromium release assay (4 h) using HeLa cells as targets and KHYG1 NK cells as effectors. (g) HeLa cells were exposed to KHYG1 NK cells at the indicated effector to target (E:T) ratio. After 5 h, cytokines in supernatants were measured by CBA. Error bars represent the mean±S.E.M. of triplicate determinations from a representative experiment, *P<0.05 by unpaired Student’s t-test

NK cells recognize tumor targets via a complex network of activating and inhibitory receptors. If activation signals dominate inhibitory signals within the immunological synapse, NK effector function is triggered.37, 38 Human KHYG1 NK cells efficiently lysed HeLa cell targets (Figure 1f), and similar to CD8+ T cells, also released TNF and IFNγ under these conditions (Figure 1g). Taken together, these data demonstrate that CLs concurrently lyse target cells and release inflammatory cytokines.

CL killing is potentiated by IAP antagonism

Recent evidence suggests that SMs function, at least in part, by enhancing anti-tumor immunity.14, 15, 18 To investigate whether SMs modulate CL killing, we performed a CD8+ T-cell killing assay in the presence or absence of birinapant. We initially used a 4 h chromium release assay, in which tumor cell killing kinetics are entirely perforin dependent. Under these conditions, we observed no modulation of killing kinetics (Figure 2a, quantitated on right). Thus, birinapant does not directly modulate perforin-dependent killing. However, CLs can kill tumors through perforin-independent mechanisms, which invoke apoptosis at a slower rate in comparison with perforin/granzyme delivery. Interestingly, over 18 h, CD8+ T-cell killing of MC38-Ova tumors was enhanced almost fivefold in the presence of birinapant compared with the control (Figure 2b, quantitated on right). Similarly, birinapant did not modulate mouse NK cell perforin-dependent killing of MC38 target cells (Figure 2c, quantitated on right). However, over 18 h, NK cell killing function was enhanced about 2.5-fold in the presence of birinapant (Figure 2d, quantitated on right). Indeed, we observed a dose-dependent increase in MC38-Ova killing in the presence of a fixed number of CD8+ T-cell or exogenously added TNF (Supplementary Figure S1a & S1b). We also observed similar results using an alternative smac mimetic BV6 (Supplementary Figure S1c & S1d). These observations also translated to a human system, where birinapant enhanced human NK cell lytic function almost sixfold (Figure 2e, quantitated on right).

Figure 2.

Figure 2

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IAP antagonism promotes TNF-dependent cytotoxic lymphocyte killing. (a) Chromium release assay (4 h) using MC38-Ova cells as targets and OT-I T cells as effectors, in the presence or absence of birinapant (1 μM). (b) Chromium release assay (18 h) using MC38-Ova cells as targets and OT-I T cells as effectors, in the presence or absence of birinapant (1 μM). (c) Chromium release assay (4 h) using MC38 cells as targets and murine NK cells as effectors, in the presence or absence of birinapant (1 μM). (d) Chromium release assay (18 h) using MC38 cells as targets and murine NK cells as effectors, in the presence or absence of birinapant (1 μM). (e) Chromium release assay (18 h) using HeLa cells as targets and KHYG1 NK cells as effectors, in the presence or absence of birinapant (1 μM). (f) Chromium release assay (18 h) using MC38-Ova or MC38-Vector cells as targets and OT-I T cells as effectors, in the presence or absence of birinapant (1 μM). (g) Chromium release assay (18 h) using MC38-Ova cells as targets and OT-I T cells as effectors, in the presence or absence of birinapant (1 μM) and anti-TNF-neutralizing antibody. (h) Chromium release assay (18 h) using MC38 cells as targets and murine NK cells as effectors, in the presence or absence of birinapant (1 μM) and anti-TNF-neutralizing antibody. (i) Chromium release assay (18 h) using MC38 cells treated with the indicated concentration of TNF, in the presence or absence of birinapant (1 μM) and emricasan (1 μM). (j) MC38-Ova cells were treated with TNF (50 ng/ml) in the presence or absence of birinapant (1 μM). At the indicated time-points proteins were analyzed by western blotting. Error bars represent the mean±S.E.M. of triplicate determinations from a representative experiment, *P<0.05 by unpaired Student’s t-test

To determine whether birinapant enhanced CL killing in a TCR-MHC-I dependent manner, we compared killing kinetics of MC38-Ova or vector-expressing cells, in the presence or absence of birinapant. In this instance, birinapant failed to enhance CD8+ T-cell killing in the absence of antigen recognition, indicating that it only promotes perforin-independent CD8+ T-cell killing upon TCR engagement by cognate antigen (Figure 2f, quantitated on right).

Perforin-independent CL killing can occur through receptor ligation on tumor cells by ligands of the TNF superfamily (TNF, TRAIL and FasL), which are expressed by CLs.39 As birinapant sensitizes to TNF-induced killing, we asked if CL-derived TNF was killing tumor cells in the presence of birinapant. To this end, we performed a killing assay in the presence or absence of a TNF-neutralizing antibody. TNF neutralization reduced CD8+ T-cell killing to ~50% of controls (Figure 2g, quantitated on right, Supplementary Figure S3), and the birinapant enhancement of killing was completely abolished under these conditions. We observed similar results with NK cells (Figure 2h, quantitated on right, Supplementary Figure S3). Inhibiting caspase activity in target cells with emricasan9 entirely abolished CD8+ T-cell killing in either the presence or absence of birinapant (Figure 2i, quantitated on right). Furthermore, we confirmed that birinapant enhanced TNF-induced apoptosis by western blot, which shows accelerated cleavage of caspase 3 and PARP (Figure 2j). Surprisingly, given the literature on Smac-mimetics, birinapant did not increase cytokine secretion from CTL upon target engagement (Supplementary Figure S2). Taken together, these data demonstrate that birinapant does not improve tumor cell killing by increasing CL TNF production but rather harnesses CL-derived TNF to drive tumor cell apoptosis, which markedly improves CL tumor killing potency.

IAP antagonism overcomes failed perforin-dependent killing

Successful perforin/granzyme-dependent tumor cell killing results in detachment of the CL via an unidentified signal generated by caspase activity in the apoptotic target cell.40 However, if a CL fails to kill a target due to perforin/granzyme deficiency, or high levels of apoptosis inhibitory proteins such as XIAP and Bcl2 family members, the absence of a detachment signal drives a prolonged synapse, resulting in inflammatory cytokine hyper-secretion.40 Thus, we asked whether antagonizing IAPs could re-route the failed perforin-dependent pathway to apoptosis to the TNF-induced apoptotic pathway. As previously described, we also observed that Pfn−/− CD8+ T cells produced significantly more TNF and IFNγ than Pfn+/+ CD8+ T cells in the presence of target cells40 (Figure 3a). Remarkably, but consistent with our hypothesis, birinapant converted defective perforin-deficient T cells into equally potent killers as their wild-type counterparts, particularly when lower ratios of T cells to target cells were used (Figure 3b, quantitated on right, Supplementary Figure S3). Pfn−/− NK cells also produced substantially more cytokines that Pfn+/+ NK cells (Figure 3c), and this also translated into effective target cell killing in the presence of birinapant (Figure 3d, quantitated on right, Supplementary Figure S3). To confirm that Pfn−/− CLs were killing via the classical TNF pathway, we exposed Pfn−/− CD8+ T cells to MC38-Ova targets and performed western blot analysis. Birinapant enhanced cleavage of capase-3 and PARP upon T-cell exposure (Figure 3e), translating to decreased survival of tumor cell targets in clonogenic assays (Figure 3f). We also detected an increased percentage of tumor cell targets staining positive for cleaved caspase 3/7 upon exposure to Pfn−/− CD8+ T cells in the presence of birinapant (Figure 3g, quantitated on right). Interestingly, we also noticed an increase in the frequency of MC38-Ova–CD8+ T-cell synapses in which the target cell was caspase 3/7 positive following birinapant treatment (Figure 3h, quantitated on right). This suggested that CD8+ T cells may be using a membrane-bound form of TNF to kill targets, which is enhanced by birinapant. Taken together, these data demonstrate that perforin is dispensable for the action of birinapant by harnessing the potent pro-apoptotic properties of TNF.

Figure 3.

Figure 3

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IAP antagonism promotes perforin-independent CL killing. (a) MC38-Ova cells were exposed to Pfn+/+ or Pfn−/− OT-I T cells. After 5 h, cytokines in supernatants were measured by CBA. (b) Chromium release assay (18 h) using MC38-Ova cells as targets and Pfn+/+ or Pfn−/− OT-I T cells, in the presence or absence of birinapant (1 μM). (c) MC38-Ova cells were exposed to Pfn+/+ or Pfn−/− murine NK cells. After 5 h, cytokines in supernatants were measured by CBA. (d) Chromium release assay (18 h) using MC38 cells as targets and Pfn+/+ or Pfn−/− murine NK cells, in the presence or absence of birinapant (1 μM). (e) MC38-Ova cells (2 × 106) were exposed to 2 × 105 Pfn−/− OT-I T cells in the presence or absence of birinapant (1 μM). TNF (50 ng/ml) and birinapant (1 μM) were used as positive controls. At the indicated time-points, proteins were analyzed by western blotting. (f) MC38-ova cells (1 × 106) were exposed to Pfn−/− OT-I T cells (2 × 105). After 48 h, cells were visualized by crystal violet staining. (g–h) MC38-Ova cells were seeded in chamber slides then overlaid with Pfn−/− OT-I T cells. After 8 h, cells were fixed, stained as indicated, then visualized by confocal microscopy. A minimum of 50 cells was counted in each condition. Error bars represent the mean±S.E.M. of triplicate determinations from a representative experiment, *P<0.05 by unpaired Student’s t test

Birinapant promotes bystander and synapse-mediated killing

To further interrogate the mechanism by which birinapant promotes CL killing at the single cell level, we performed time-lapse microscopy. We labeled perforin-deficient OT-1 T cells (to eliminate rapid granzyme-dependent apoptosis) with Cell Trace Violet (CTV) and monitored their ability to synapse with, and kill MC38-Ova cells. Induction of tumor cell apoptosis was evident by addition of a fluorescent cleaved caspase 3/7 reporter to the assay medium. Figure 4a shows a series of still images captured from Supplementary Movie 1 (supplementary data) in which the T cells, in the presence of DMSO, fail to kill any of the tumor cell targets over an 8 h period. However, in the presence of birinapant, target cell apoptosis can be detected by 6 h, and is widespread by 8 h after exposure to T cells (Figure 4a, and supplementary Movie 2). To interrogate the contribution of soluble TNF in mediating bystander killing, we evaluated the number of tumor cells that underwent apoptosis in the absence of a T-cell synapse. Interestingly, we observed a significant increase in bystander killing in the presence of birinapant (Figure 4b). Furthermore, we found that birinapant increased the frequency of killing that occurred during an immune synapse, prior to CL detachment (Figure 4c).

Figure 4.

Figure 4

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Live imaging reveals that birinapant promotes bystander and TNF-mediated synaptic killing. (a) MC38-Ova cells were seeded in chamber slides, then overlaid with perforin-deficient OT-I T cells. T cells were labeled with cell trace violet (CTV). A cleaved caspase 3/7 reporter and birinapant (1 μM)/DMSO was added to the co-culture media. Representative still images at the indicated time-points are depicted (hr:min). (b) MC38-Ova cells that became caspase 3/7 positive in the absence of an immune synapse with a T-cell within the 8 h time span. (c) MC38-Ova cells that became caspase 3/7 positive during an immune synapse with a T-cell within the 8 h time span. All quantification data are pooled from five separate camera positions across two individual experiments. Error bars represent the mean±S.E.M *P<0.05 by unpaired Student’s t-test

TNF is expressed on the membrane of CLs where it is processed and shed from the membrane by the matrix metalloprotease, TACE.41 Because we observed enhanced CL killing upon immune synapse formation in the presence of birinapant (Figure 4c), we asked if membrane-bound TNF contributed to CL-mediated tumor killing upon treatment. Initially we used the NK cell line, KHYG1, owing to its robust production of readily detectable amounts of TNF. PMA/Ionomycin induced upregulation of membrane-bound TNF, which was detected by flow cytometry, and as expected, the amount of membrane TNF was further increased by TACE inhibition using marimastat (Figure 4d). To determine whether NK cells could kill targets via membrane TNF we used Pfn−/− murine NK cells. A TNF-neutralizing antibody (which blocks soluble and membrane TNF) reduced the cytotoxicity of Pfn−/− NK cells to negligible levels (Figure 4e). Although marimastat entirely blocked release of soluble TNF into the supernatant upon tumor cell engagement (Figure 4f) it did not phenocopy the complete block on NK cell killing with anti-TNF (Figure 4g). This suggests that both soluble and membrane-bound forms of TNF contribute to NK cell cytotoxicity in this context. Furthermore, NK cell killing via membrane TNF (due to marimastat treatment) could be significantly enhanced in the presence of birinapant (Figure 4h, quantitated on right). Thus, birinapant is able to harness both membrane-bound and soluble forms of TNF to kill tumor cells.

PD-L1 suppresses secretion of TNF and cell killing by CL

Checkpoint blockade therapy is thought to reverse T-cell exhaustion by re-instating cytotoxic function and cytokine secretion.30, 31, 37 Although it has been demonstrated that anti-PD-1 therapy can elevate IFNγ secretion by CD8+ T cells,36, 42 fewer studies have focused on the implications of elevated TNF secretion upon PD-L1/PD-1 disruption.36 Because we had shown that birinapant potentiates CL function through TNF, we investigated whether the PD-1/PD-L1 axis modulated TNF secretion by CD8+ T cells. As expected, IFNγ upregulated PD-L1 and MHC-I expression on MC38-ova cells (Figure 5a). Indeed, elevated MHC-I expression increased the efficiency of T-cell-mediated killing (Figure 5b, quantitated on right). However, under these conditions, neutralization of PD-1 enhanced T-cell killing even further, indicating that PD-L1 was suppressing T-cell effector function (Figure 5c, quantitated). To interrogate the role of PD-L1 in the absence of concurrent MHC-I regulation, we stably expressed PD-L1 on MC38-Ova cells (MC38PD-L1), (Figure 5d). Initially, we monitored cytokine production in the presence of a control antibody, or a PD-1 neutralizing antibody. Importantly, antagonizing PD-1 leads to significantly higher production of TNF and IFNy, which was amplified in the absence of perforin (Figure 5e,Supplementary Figure S3). Blockade of PD-1 also increased killing of MC38PD-L1 cells by T cells (Figure 5f, quantitated on right). Importantly, this also occurred in the absence of perforin, demonstrating the importance of enhanced cytokine production in checkpoint blockade anti-tumor action. Taken together, PD-1 ligation by tumor cell expressed PD-L1 suppresses T-cell effector function (cytokine production and target cell death).

Figure 5.

Figure 5

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PD-L1 inhibits T-cell secretion of TNF and cytotoxicity. (a) MC38-Ova cells were left untreated or treated with IFNγ (5 ng/ml) for 18 h, then flow cytometry was used to analyze expression of MHC-I (H-2Kb) and PD-L1. (b) MC38-Ova cells were left untreated or treated with IFNγ (5 ng/ml) for 18 h, then used as targets in a chromium release assay with OT-I T cells (18 h). (c) MC38-Ova cells were left untreated or treated with IFNγ (5 ng/ml) for 18 h, then used as targets in a chromium release assay with OT-I T cells (18 h), in the presence of control antibody or anti-PD-1. (d) Flow cytometric analysis of MC38-Ova cells transduced with vector, PD-L1, or treated with IFNγ (5 ng/ml for 18 h). (e) MC38PD-L1 cells (1 × 105) were exposed to Pfn+/+ or Pfn−/− OT-I T cells (1 × 104), in the presence of control antibody or anti-PD-1 (50 μg/ml). After 48 h, cytokines in supernatants were measured by CBA. (f) MC38PD-L1 cells (1 × 105) were exposed to Pfn+/+ or Pfn−/− OT-I T cells (1 × 104), in the presence of control antibody or anti-PD-1 (50 μg/ml). After 48 h, survival was analyzed by PI staining and flow cytometry. Error bars represent the mean±S.E.M. of triplicate determinations from a representative experiment, *P<0.05 by unpaired Student’s t-test

Inhibition of IAPs by birinapant increases perforin-independent tumor cell killing by CLs, which is further enhanced by co-inhibition of PD-L1

Because we had demonstrated that birinapant sensitized tumor cells to CL-derived TNF (Figure 2), and tumor cells suppress TNF production through PD-1 ligation (Figure 5), we asked if co-inhibition of immune checkpoint could enhance the efficacy of birinapant. Anti-PD-1 or birinapant treatment alone decreased the survival of MC38PD-L1 cells upon T-cell exposure. However, the combination of these two agents reduced the viability of target cells to just 28 percent (Figure 6a, quantitated on right). Importantly, we also observed a dramatic decrease in tumor cell survival using Pfn−/− T cells. Thus, effective tumor cell killing upon inhibition of IAPs and PD-L1 occurs in a perforin-independent, TNF-dependent fashion. We also used a chromium release assay to examine the killing kinetics upon co-inhibition. Although anti-PD-1 or birinapant alone enhanced T-cell killing kinetics, combining these two agents resulted in markedly enhanced killing (Figure 6b). Again, we observed the same level of killing in the complete absence of perforin (Figure 6c).

Figure 6.

Figure 6

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Birinapant increases tumor cell death by cytotoxic lymphocytes, which is further enhanced by PD-1 blockade. (a) MC38PD-L1 cells were exposed to Pfn+/+ or Pfn−/− OT-I T cells, in the presence or absence of DMSO/birinapant (1 μM) and/or control/anti-PD-1 antibody (10 μg/ml). After 48 h, survival was analyzed by PI staining and flow cytometry. (b) Chromium release assay (18 h) using MC38PD-L1 cells as targets and Pfn+/+ OT-I T cells as effectors, in the presence or absence of DMSO/birinapant (1 μM) and/or control/anti-PD-1 antibody (50 μg/ml). (c) Chromium release assay (18 h) using MC38PD-L1 cells as targets and Pfn−/− OT-I T cells as effectors, in the presence or absence of DMSO/birinapant (1 μM) and/or control/anti-PD-1 antibody (50 μg/ml). Error bars represent the mean±S.E.M. of triplicate determinations from a representative experiment, *P<0.05 by unpaired Student’s t-test

Discussion

Although it is widely accepted that IAP antagonists promote tumor cell death by sensitizing them to TNF-induced apoptosis, the majority of studies that demonstrate this phenomenon have been conducted in vitro. Cell lines that produce TNF in response to SMs are usually sensitive to these agents as a mono-therapy due to autocrine/paracrine TNF signaling.6, 7, 8 However, those cells that fail to produce TNF upon IAP loss can often be killed by exogenous addition of TNF in order to invoke apoptosis.6, 7, 8 The mechanisms by which Smac-mimetics display anti-tumor efficacy in vivo are therefore likely to be complex. First, there are multiple immune cell subtypes, particularly CLs and monocytes/macrophages, that produce TNF and could supply the TNF required for SM anti-tumor efficacy in vivo. This is evident from studies demonstrating that SM synergize with inflammatory adjuvants, which trigger a systemic increase in TNF levels, which is released by responder cells of the innate immune system.15, 18 Second, there is emerging evidence that IAP antagonists promote anti-tumor immunity independently from direct induction of tumor cell apoptosis.14 For example, the IAP antagonist, compound A, has been shown to inhibit tumor growth through inflammatory destruction of tumor vasculature.43 This occurred using tumor cells (murine melanoma B16 cells) that are completely refractory to TNF/SM-induced cell death. Furthermore, antagonizing IAPs appears to promote anti-tumor immunity through enhancing effector T-cell co-stimulation and proliferation. This occurs through triggering alternative, NIK-dependent NF-κB activation in response to antigen or CD3/CD28 stimulation.44 Together, these studies highlight that the immune system may be utilized to improve the efficacy of SMs; however, a better understanding of how SMs modulate tumor immunity is required to take full advantage of this opportunity.

Many diverse solid tumor types are heavily infiltrated with CLs, and this often predicts patient prognosis.22 Therefore, harnessing these cells as vehicles to deliver TNF to the tumor site may be very effective therapeutically. Indeed, CD8+ T cells trigger TNF synthesis upon tumor antigen recognition, avoiding robust TNF production in non-malignant tissue. Similarly, NK cells produce robust quantities of TNF upon ligation of activating receptors through tumor-expressed ligands. However, how SMs modulate the killing capacity of these cells remains unexplored. Here we demonstrated that CL-mediated killing of tumor cells is dramatically improved in the presence of SM. This occurs through TNF production upon antigen recognition or NK-activating receptor ligation. Importantly, we found that tumor cells suppress this route to their demise by dampening T-cell TNF production through expression of PD-L1. Furthermore, our data demonstrating that PD-1 blockade/IAP antagonism improves CL killing in the complete absence of perforin, highlights the importance of cytokines in the efficacy of immune checkpoint therapy (Figure 7).

Figure 7.

Figure 7

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Schematic representation of pathways leading to cytotoxic lymphocyte killing upon IAP or PD-1 antagonism. Ligation of TCR with cognate tumor antigen or NK cell activating receptor stimulation drives TNF production. PD-L1 expression on tumor cells, dampens signaling cascades that drive TNF production and perforin/granzyme-mediated apoptosis. Furthermore, tumor cell expression of cIAP1/2 protects them from apoptosis mediated by TNF released from CLs. Thus co-inhibition of PD-L1 and IAPs increases TNF production from CLs, and decreases the TNF threshold for apoptosis induction

Much attention has been given to the anti-tumor efficacy of checkpoint blockade therapy, which is mediated by T cells. However, sporadic reports suggest that the function of NK cells is also enhanced via a similar mechanism.45 Thus, such agents, particularly when combined with SMs, may promote NK cell-dependent anti-tumor immunity. Certainly, biologics that specifically enhance NK cell cytokine production could be potently enhance the efficacy of SM. This type of treatment is likely to be effective against hematological tumors, which are characterized by suppressed NK cell effector function prior to chemotherapy or allogeneic stem cell plantation.46, 47 NK cells can also lyse tumor cells through the TRAIL/Fas pathway, independently of perforin. Because it is known that SMs can greatly sensitize tumor cells to death mediated by TRAIL or Fas ligand,48 antagonizing IAPs may improve this pathway in tumors that express DR4/5 or CD95. More studies will be required to understand the role of these death ligands in the efficacy of SMs in vivo.

Taken together, we have demonstrated that IAPs and PD-L1 protect tumor cells from the pro-apoptotic function of TNF derived from CLs. Thus, co-inhibition of these molecules markedly sensitizes tumor cells to killing by CLs (Figure 7). We believe the present data warrant further evaluation of these drugs in treating diverse cancer types. Importantly, clinical trials in which SMs will be administered in combination with immune checkpoint blockade are soon to commence (ClinicalTrials.gov: NCT02587962, NCT02890069). Thus, our data provide important information regarding the mode of action upon combining these agents in patients, and provide an opportunity to improve their efficacy through mechanistic understanding.

Materials and Methods

The cell lines MC38 and HeLa were cultured in RPMI medium (GIBCO, Melbourne, Victoria, Australia; Invitrogen, Scoresby, Victoria, Australia) supplemented with 10% FCS (Thermo Scientific, Scoresby, Victoria, Australaia), penicillin/streptomycin (Gibco), and incubated at 37 °C in 5% CO2. Primary NK cells were isolated from C57Bl/6 splenocytes, using a negative selection isolation kit (Stem Cell Technologies, Tullamarine, Victoria, Australia). Murine NK cells were cultured in RPMI medium supplemented with 10% fetal calf serum, l-glutamine, penicillin/streptomycin, non-essential amino acids, sodium pyruvate, HEPES, β-2-mercaptoethanol (Calbiochem, Macquarie Park, New South Wales, Australia), and 1000 IU/ml recombinant human IL-2 (ROCHE, Sydney, New South Wales, Australia), at 37 °C in a 5% CO2. Primary CLs were isolated from the spleens of C57Bl/6.OT-I or OT-I.Prf−/− mice and activated with OVA257 peptide. Activated CLs were generally used on day 5–10. CLs were cultured as murine NK cells, with 100 IU/ml.

Cytotoxic assays

CL activity was measured using a standard chromium release assay as follows: of 1 × 106 targets were labeled with 100 μCi of 51Cr (Perkin Elmer, Melbourne, Victoria, Australia) and added to V bottom plates. CLs cells were then added to the targets at the indicated effector to target (E:T) ratios. After 4 or 18 h at 37 °C in 5% CO2, supernatants were harvested, and the level of 51Cr was quantified by a gamma counter (Wallac Wizard). Percentage specific killing was determined using the formula: (Sample 51Cr release – Spontaneous 51Cr release)/(Total 51Cr release – Spontaneous 51Cr release) × 100, and represented as a Michaelis–Menten kinetic trend. All assays were performed using triplicate wells. The rate of killing in the control is represented as 100% killing and treatment groups compared with this value.

Antibodies

Neutralizing antibodies: anti-TNF (Biolegend, San Diego, CA, USA, clone MP6-XT22), anti-PD-1 (BioXCell, Lebanon, NH, USA, clone Rpmi-14). Antibodies for western blot: anti caspase 3, anti-PARP, anti cIAP1 (Cell Signaling, Danvers, MA, USA), anti tubulin (Rockland, Limerick, PA, USA). Antibodies for immunofluorescence: anti-TNF (Biolegend, clone MP6-XT22), IFNγ (eBiosciences, Scoresby, Victoria, Australia, clone XMG1.2), Tubulin (Rockland). Rhodamine-Phalloidin was used to detect actin. Secondary antibodies conjugated to Alexa fluorophores and ProLong antifade with DAPI were purchased from Molecular Probes (Scoresby, Victoria, Australia).

Cytokine bead array (CBA)

Cytokines were detected using a mouse inflammation CBA kit (BD Biosciences, 552364) as per manufacturer's instructions. Beads were analyzed on a FACS Verse (BD Biosciences, North Ryde, New South Wales, Australia). All assays were analyzed using triplicate determinations.

Immunofluorescent fixed microscopy

Cells were adhered onto eight-well chamber slides overnight, prior to addition of effector cells for the indicated time periods. Cells were then fixed, permeabilized, and labeled with primary Abs, followed by detection with Alexa Fluor–conjugated secondary Abs and mounted in Prolong antifade containing DAPI (Molecular Probes). The slides were imaged using a FV1000 confocal microscope (Olympus, NY, USA). CLs selected for quantitation had a single contact site with one tumor cell, indicating a single synapse event. The percentage of T cells that were positive for the indicated marker (cytokines or caspase positivity), in a synapse or not, was quantitated manually by confocal microscopy. A minimum of 100 cells was counted in each condition.

Time-lapse microscopy

MC38-Ova cells were seeded into each well of an eight-well chamber slide (Ibidi, Munich, Germany) and incubated overnight at 37°C/10% CO2. OT-I T cells were labeled with CTV (Molecular Probes), then added to adherent targets, in media containing caspase 3/7 detection reagent (ThermoFischer). Chamber slides were mounted on a heated stage within a temperature-controlled chamber maintained at 37°C, and constant CO2 concentrations (5 or 7%) were infused using a gas incubation system with active gas mixer ('The Brick' Ibidi). Optical sections were acquired through sequential scans or Brightfield/DIC on a TCS SP5 confocal microscope (Leica Microsystems, Deerfield, IL, USA) using a × 40 (NA 0.85) air objective and Leica LAS AF software. Image analysis was performed using Meta-Morph Imaging Series 7 software (Universal Imaging, Downingtown, PA, USA).

Acknowledgments

The work was supported by the National Health and Medical Research Council of Australia (NHMRC), grants 1081376 and 1079319; fellowships 1058190 to CJK and 1107149 to JS and the WWCR grant 15-0042 to NL. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS (9000220).

Footnotes

Supplementary Information accompanies this paper on Cell Death and Differentiation website (http://www.nature.com/cdd)

Edited by T Mak

John Silke was previously a member of the TetraLogic scientific advisory board. The remaining authors declare no conflict of interest.

Supplementary Material

Supplementary Figures and Movie Legend
Click here for additional data file. (241.6KB, pdf)
Supplementary movie 1
Click here for additional data file. (254.4MB, avi)
Supplementary movie 2
Click here for additional data file. (419.5MB, avi)

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Associated Data

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Supplementary Materials

Supplementary Figures and Movie Legend
Click here for additional data file. (241.6KB, pdf)
Supplementary movie 1
Click here for additional data file. (254.4MB, avi)
Supplementary movie 2
Click here for additional data file. (419.5MB, avi)

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