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. 2017 Aug 22;7(14):3595–3607. doi: 10.7150/thno.18974

Figure 2.

Figure 2

Gint4.T aptamer inhibits PDGFRβ-mediated downstream signaling pathway in BM-MSCs. (A) Lysates from U87MG, MCF-7 and BM-MSCs were analyzed by immunoblot with anti-PDGFRβ antibody, and equal loading was confirmed by immunoblot with anti-actin antibody (left). PDGFRβ mRNA levels were analyzed by Real Time-PCR (right). (B) BM-MSCs cells were serum-starved for 18 hours and then left untreated or stimulated for 15 minutes with 100 ng/ml PDGF-BB in the absence or in the presence of 200 nmol/l Gint4.T or Scr, as indicated. Cell lysates were immunoblotted with anti-pPDGFRβ, anti-pERK, anti-pAkt antibodies. Filters were stripped and reprobed with anti-PDGFRβ, anti-Erk and anti-Akt antibodies, as indicated. Equal loading was confirmed by immunoblot with anti-vinculin antibody. The histogram indicates pPDGFRβ/PDGFRβ, pERK/ERK and pAKT/AKT ratio of densitometric signals, reported as relative to Scr-treated cells, arbitrarily set to 1. Depicted results represent one of three typical experiments performed. Molecular weights of indicated proteins are reported (A and B).