FIG 4 .

Activation of shp and the downstream regulon with and without sSHP in S. pneumoniae D39 and the Δrgg (SP68) and Over-EXP (SP44) mutant strains. shp gene expression was determined by RT-PCR. Cultures were grown in C+Y and incubated at 37°C for 2 h at a starting OD₆₀₀ of 0.05 with (as indicated by a plus sign) or without 1 µM S. pneumoniae sSHP prior to RNA extraction. The y axis shows relative mRNA expression (dRn) on a log₁₀ scale. Each bar represents mean values with the standard error of the mean. Panels: A, shp; B, SPD_0940; C, SPD_0947; D, SPD_0950; E, SPD_0952.